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Literature summary extracted from

  • Albi, T.; Serrano, A.
    Two exopolyphosphatases with distinct molecular architectures and substrate specificities from the thermophilic green-sulfur bacterium Chlorobium tepidum TLS (2014), Microbiology, 160, 2067-2078.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.11 expression in Escherichia coli Chlorobaculum tepidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.11 EDTA
-
 Chlorobaculum tepidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.11 0.0977
-
 (phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 0.213
-
 (phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 0.242
-
 guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 0.264
-
 (phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 0.335
-
 guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 0.597
-
 (phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.11 K+ 20 mM, about 3fold stimulation Chlorobaculum tepidum
3.6.1.11 Mg2+ best activator, maximum activity at 5 mM Chlorobaculum tepidum
3.6.1.11 Mn2+ 5 mM, about 37% of the activity with Mg2+ Chlorobaculum tepidum
3.6.1.11 Mn2+ 5 mM, about 65% of the activity with Mg2+ Chlorobaculum tepidum
3.6.1.11 additional information presence of divalent cation is absolutely required, Mg2+ is preferred Chlorobaculum tepidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.11 37000
-
 1 * 37000, SDS-PAGE Chlorobaculum tepidum
3.6.1.11 38800
-
 gel filtration Chlorobaculum tepidum
3.6.1.11 59900
-
 2 * 59900, SDS-PAGE Chlorobaculum tepidum
3.6.1.11 100400
-
 gel filtration Chlorobaculum tepidum

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.11 Chlorobaculum tepidum Q8KBS0
-
-
3.6.1.11 Chlorobaculum tepidum Q8KG69
-
-
3.6.1.11 Chlorobaculum tepidum DSM 12025 Q8KBS0
-
-
3.6.1.11 Chlorobaculum tepidum DSM 12025 Q8KG69
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.11 15
-
 substrate (phosphate)300, pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 126
-
 substrate (phosphate)300, pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 170
-
 substrate (phosphate)13-18, pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 180
-
 substrate (phosphate)13-18, pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 590
-
 substrate (phosphate)3, pH 6.5, 25°C Chlorobaculum tepidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.11 (phosphate)13-18 + H2O
-
 Chlorobaculum tepidum (phosphate)12-17 + phosphate
-
 ?
3.6.1.11 (phosphate)13-18 + H2O
-
 Chlorobaculum tepidum DSM 12025 (phosphate)12-17 + phosphate
-
 ?
3.6.1.11 (phosphate)3 + H2O
-
 Chlorobaculum tepidum (phosphate)2 + phosphate
-
 ?
3.6.1.11 (phosphate)3 + H2O best substrate Chlorobaculum tepidum (phosphate)2 + phosphate
-
 ?
3.6.1.11 (phosphate)3 + H2O
-
 Chlorobaculum tepidum DSM 12025 (phosphate)2 + phosphate
-
 ?
3.6.1.11 (phosphate)3 + H2O best substrate Chlorobaculum tepidum DSM 12025 (phosphate)2 + phosphate
-
 ?
3.6.1.11 (phosphate)300 + H2O or even longer chain length Chlorobaculum tepidum (phosphate)299 + phosphate
-
 ?
3.6.1.11 (phosphate)300 + H2O or even longer chain length Chlorobaculum tepidum DSM 12025 (phosphate)299 + phosphate
-
 ?
3.6.1.11 guanosine 5'-tetraphosphate + H2O
-
 Chlorobaculum tepidum GTP + phosphate
-
 ?
3.6.1.11 guanosine 5'-tetraphosphate + H2O
-
 Chlorobaculum tepidum DSM 12025 GTP + phosphate
-
 ?
3.6.1.11 additional information isoform Ppx1 shows substantial nucleoside triphosphatase activity Chlorobaculum tepidum ?
-
 ?
3.6.1.11 additional information isoform Ppx1 shows substantial nucleoside triphosphatase activity Chlorobaculum tepidum DSM 12025 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.11 dimer 2 * 59900, SDS-PAGE Chlorobaculum tepidum
3.6.1.11 monomer 1 * 37000, SDS-PAGE Chlorobaculum tepidum

Synonyms

EC Number Synonyms Comment Organism
3.6.1.11 CT0099
-
 Chlorobaculum tepidum
3.6.1.11 CT1713
-
 Chlorobaculum tepidum
3.6.1.11 PPX1
-
 Chlorobaculum tepidum
3.6.1.11 PPX2
-
 Chlorobaculum tepidum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.11 13.5
-
 (phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 14.2
-
 guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 157
-
 (phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 308
-
 guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 399
-
 (phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 458
-
 (phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.11 6.5
-
-
 Chlorobaculum tepidum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.11 5.5 7.5 more than 30-40% of maximum activity Chlorobaculum tepidum
3.6.1.11 10
-
 about 90% of maximum activity Chlorobaculum tepidum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.11 42
-
 guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 63
-
 (phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 263
-
 (phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 1273
-
 guanosine 5'-tetraphosphate pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 1716
-
 (phosphate)13-18 pH 6.5, 25°C Chlorobaculum tepidum
3.6.1.11 4081
-
 (phosphate)3 pH 6.5, 25°C Chlorobaculum tepidum