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Literature summary extracted from
- Characterization and functional cloning of an aromatic nitrilase from Pseudomonas putida CGMCC3830 with high conversion efficiency toward cyanopyridine (2013), J. Mol. Catal. B, 97, 175-183.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.5.1 | expressed in Escherichia coli Rosetta-gami (DE3) cells | Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.5.1 | Ag+ | complete inhibition at 1 mM | Pseudomonas putida |  |
| 3.5.5.1 | ascorbic acid | 68% residual activity at 1 mM | Pseudomonas putida | |
| 3.5.5.1 | Co2+ | 44.9% residual activity at 1 mM | Pseudomonas putida | |
| 3.5.5.1 | Cu2+ | 19.7% residual activity at 1 mM | Pseudomonas putida | |
| 3.5.5.1 | EDTA | 93.2% residual activity at 1 mM | Pseudomonas putida | |
| 3.5.5.1 | Fe2+ | 75.4% residual activity at 1 mM | Pseudomonas putida | |
| 3.5.5.1 | Fe3+ | 53.3% residual activity at 1 mM | Pseudomonas putida | |
| 3.5.5.1 | Hg+ | complete inhibition at 1 mM | Pseudomonas putida | |
| 3.5.5.1 | iodoacetamide | 56.6% residual activity at 1 mM | Pseudomonas putida | |
| 3.5.5.1 | Mn2+ | 67.4% residual activity at 1 mM | Pseudomonas putida | |
| 3.5.5.1 | additional information | not inhibited by L-glutathione, L-cysteine, dithiothreitol, and 8-hydroxyquinoline | Pseudomonas putida | |
| 3.5.5.1 | N-ethylmaleimide | 57.3% residual activity at 1 mM | Pseudomonas putida | |
| 3.5.5.1 | Ni2+ | 21.6% residual activity at 1 mM | Pseudomonas putida | |
| 3.5.5.1 | Zn2+ | 57% residual activity at 1 mM | Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.5.1 | 27.9 | - |
3-Cyanopyridine | at pH 7.5 and 30°C | Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 41000 | - |
8 * 41000, SDS-PAGE | Pseudomonas putida |
| 3.5.5.1 | 310000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.1 | 3-cyanopyridine + 2 H2O | Pseudomonas putida | - |
nicotinic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-cyanopyridine + 2 H2O | Pseudomonas putida CGMCC3830 | - |
nicotinic acid + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.5.1 | Pseudomonas putida | - |
- |
- |
| 3.5.5.1 | Pseudomonas putida CGMCC3830 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.5.1 | ammonium sulfate precipitation, butyl-Sepharose column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.1 | 2-chloro-4-cyanopyridine + H2O | 75.9% activity compared to 3-cyanopyridine | Pseudomonas putida | ? | - |
? | |
| 3.5.5.1 | 2-cyanopyridine + 2 H2O | 1.5% activity compared to 3-cyanopyridine | Pseudomonas putida | pyridine 2-carboxylic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-cyanopyridine + 2 H2O | - |
Pseudomonas putida | nicotinic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-cyanopyridine + 2 H2O | 100% activity | Pseudomonas putida | nicotinic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-cyanopyridine + 2 H2O | - |
Pseudomonas putida CGMCC3830 | nicotinic acid + NH3 | - |
? | |
| 3.5.5.1 | 4-cyanopyridine + 2 H2O | 79.5% activity compared to 3-cyanopyridine | Pseudomonas putida | pyridine 4-carboxylic acid + NH3 | - |
? | |
| 3.5.5.1 | 4-methoxyphenylacetonitrile + H2O | 0.6% activity compared to 3-cyanopyridine | Pseudomonas putida | 4-methoxyphenylacetic acid + NH3 | - |
? | |
| 3.5.5.1 | acetonitrile + H2O | 1.0% activity compared to 3-cyanopyridine | Pseudomonas putida | acetic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | 17.2% activity compared to 3-cyanopyridine | Pseudomonas putida | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | acrylonitrile + H2O | 17.2% activity compared to 3-cyanopyridine | Pseudomonas putida CGMCC3830 | acrylic acid + NH3 | - |
? | |
| 3.5.5.1 | aminoacetonitrile + H2O | 9.0% activity compared to 3-cyanopyridine | Pseudomonas putida | aminoacetic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | 70.4% activity compared to 3-cyanopyridine | Pseudomonas putida | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | 70.4% activity compared to 3-cyanopyridine | Pseudomonas putida CGMCC3830 | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | beta-aminopropionitrile + H2O | 8.6% activity compared to 3-cyanopyridine | Pseudomonas putida | beta-aminopropionic acid + NH3 | - |
? | |
| 3.5.5.1 | iminodiacetonitrile + 2 H2O | 27% activity compared to 3-cyanopyridine | Pseudomonas putida | iminodiacetic acid + 2 NH3 | - |
? | |
| 3.5.5.1 | iminodiacetonitrile + 2 H2O | 27% activity compared to 3-cyanopyridine | Pseudomonas putida CGMCC3830 | iminodiacetic acid + 2 NH3 | - |
? | |
| 3.5.5.1 | additional information | no activity with isopentanenitrile, adiponitrile, 2-chloro-3-cyanopyridine, and mandelonitrile | Pseudomonas putida | ? | - |
? | |
| 3.5.5.1 | additional information | no activity with isopentanenitrile, adiponitrile, 2-chloro-3-cyanopyridine, and mandelonitrile | Pseudomonas putida CGMCC3830 | ? | - |
? | |
| 3.5.5.1 | pentanenitrile + H2O | 1.6% activity compared to 3-cyanopyridine | Pseudomonas putida | ? | - |
? | |
| 3.5.5.1 | pyrazinecarbonitrile + H2O | 32.7% activity compared to 3-cyanopyridine | Pseudomonas putida | ? | - |
? | |
| 3.5.5.1 | succinonitrile + H2O | 21.74% activity compared to 3-cyanopyridine | Pseudomonas putida | succinic acid + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.5.1 | homooctamer | 8 * 41000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 40 | - |
- |
Pseudomonas putida |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 30 | 50 | more than 70% activity between 30 and 50°C. A reaction temperature of 55°C leads to almost totally inactivation of the enzyme | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 7.5 | - |
- |
Pseudomonas putida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 7 | 8 | more than 80% activity between pH 7.0 and 8.0 | Pseudomonas putida |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.5.5.1 | Pseudomonas putida | isoelectric focusing | - |
5.2 |
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