EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.5.5 | expressed in Escherichia coli JM109 cells | Aspergillus niger |
3.5.5.5 | expressed in Escherichia coli JM109 cells | Neurospora crassa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.5.5 | H165N | the mutant exhibits a decreased activity for (R,S)-2-phenylpropionitrile compared to the wild type enzyme | Aspergillus niger |
3.5.5.5 | H170N | the mutant exhibits a decreased activity for (R,S)-2-phenylpropionitrile compared to the wild type enzyme | Neurospora crassa |
3.5.5.5 | W163A | the mutant exhibits a decreased activity for (R,S)-2-phenylpropionitrile compared to the wild type enzyme | Aspergillus niger |
3.5.5.5 | W168A | the mutant forms significantly increases amounts of mandelamide and 2-phenylpropionamide and demonstrates an almost complete inversion of enantioselectivity for the conversion of (R,S)-2-phenylpropionitrile (from R- to S-selectivity) | Neurospora crassa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.5 | Aspergillus niger | A2R6M7 | - |
- |
3.5.5.5 | Aspergillus niger CBS 513.88 | A2R6M7 | - |
- |
3.5.5.5 | Neurospora crassa | V5IPE4 | - |
- |
3.5.5.5 | Neurospora crassa DSM 1257 | V5IPE4 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.5 | (R,S)-2-phenylpropionitrile + 2 H2O | the enzyme exhibits a low degree of enantioselectivity toward this substrate (e.e. 35% for the (R)-acid at 35% conversion) | Neurospora crassa | 2-phenylpropionic acid + NH3 | - |
? | |
3.5.5.5 | (R,S)-2-phenylpropionitrile + 2 H2O | the enzyme produces (R)-2-phenylpropionic acid with an e.e.-value of 90% at 28% conversion | Aspergillus niger | 2-phenylpropionic acid + NH3 | - |
? | |
3.5.5.5 | (R,S)-2-phenylpropionitrile + 2 H2O | the enzyme exhibits a low degree of enantioselectivity toward this substrate (e.e. 35% for the (R)-acid at 35% conversion) | Neurospora crassa DSM 1257 | 2-phenylpropionic acid + NH3 | - |
? | |
3.5.5.5 | (R,S)-2-phenylpropionitrile + 2 H2O | the enzyme produces (R)-2-phenylpropionic acid with an e.e.-value of 90% at 28% conversion | Aspergillus niger CBS 513.88 | 2-phenylpropionic acid + NH3 | - |
? | |
3.5.5.5 | (R,S)-mandelonitrile + 3 H2O | the end product of (R,S)-mandelonitrile transformation at pH 5.0 consists of 40% mandelamide (relative to the total amount of products formed). It is observed that the production of mandelamide is delayed compared to the production of mandelic acid | Neurospora crassa | mandelic acid + mandelamide + NH3 | - |
? | |
3.5.5.5 | (R,S)-mandelonitrile + 3 H2O | the enzyme produces almost no mandelamide at pH 5 (Fig. 1a), while the R-acid is formed with a high enantiomeric excess | Aspergillus niger | mandelic acid + mandelamide + NH3 | - |
? | |
3.5.5.5 | (R,S)-mandelonitrile + 3 H2O | the end product of (R,S)-mandelonitrile transformation at pH 5.0 consists of 40% mandelamide (relative to the total amount of products formed). It is observed that the production of mandelamide is delayed compared to the production of mandelic acid | Neurospora crassa DSM 1257 | mandelic acid + mandelamide + NH3 | - |
? | |
3.5.5.5 | (R,S)-mandelonitrile + 3 H2O | the enzyme produces almost no mandelamide at pH 5 (Fig. 1a), while the R-acid is formed with a high enantiomeric excess | Aspergillus niger CBS 513.88 | mandelic acid + mandelamide + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.5.5 | Arylacetonitrilase | - |
Aspergillus niger |
3.5.5.5 | Arylacetonitrilase | - |
Neurospora crassa |
3.5.5.5 | NIT | - |
Aspergillus niger |
3.5.5.5 | NIT | - |
Neurospora crassa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 7 | - |
- |
Aspergillus niger |
3.5.5.5 | 7 | - |
- |
Neurospora crassa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.5 | 4.5 | - |
about 45% activity at pH 4.5 compared to the pH optimum at 7.0 | Aspergillus niger |
3.5.5.5 | 4.5 | - |
about 45% activity at pH 4.5 compared to the pH optimum at 7.0 | Neurospora crassa |
3.5.5.5 | 5 | - |
about 70% activity at pH 5.0 compared to the pH optimum at 7.0 | Aspergillus niger |
3.5.5.5 | 5 | - |
about 70% activity at pH 5.0 compared to the pH optimum at 7.0 | Neurospora crassa |