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Literature summary extracted from

  • Meesapyodsuk, D.; Chen, Y.; Ng, S.H.; Chen, J.; Qiu, X.
    Metabolic engineering of Pichia pastoris to produce ricinoleic acid, a hydroxy fatty acid of industrial importance (2015), J. Lipid Res., 56, 2102-2109.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.18.4 synthesis coexpression of fatty acid hydroxylase FAH and diacylglycerol acyl transferase DGAT1 in Pichia pastoris produces higher lipid contents and ricinoleic acid levels than expression of FAH alone. Coexpression in a mutant haploid strain defective in the DELTA12 desaturase activity results in a higher level of ricinoleic acid than that in the diploid strain. The ricinoleic acid produced is mainly distributed in the neutral lipid fractions, particularly the free fatty acid form, but with little in the polar lipids Claviceps purpurea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.4 expression in Pichia pastoris Claviceps purpurea

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.4 Claviceps purpurea B4YQU1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.4 oleic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Claviceps purpurea ricinoleic acid + 2 ferricytochrome b5 + H2O
-
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Synonyms

EC Number Synonyms Comment Organism
1.14.18.4 FAH
-
Claviceps purpurea