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Literature summary extracted from

  • Cho, M.; Park, H.; Park, J.; Lee, S.; Bhoo, S.; Hahn, T.
    Characterization of regiospecific flavonoid 3'/5'-O-methyltransferase from tomato and its application in flavonoid biotransformation (2012), J. Korean Soc. Appl. Biol. Chem., 55, 749-755.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.267 expression in Escherichia coli Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.267 0.00057
-
 quercetin pH 7.5, 30°C Solanum lycopersicum
2.1.1.267 0.00074
-
 luteolin pH 7.5, 30°C Solanum lycopersicum
2.1.1.267 0.0224
-
 eriodictyol pH 7.5, 30°C Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.267 Solanum lycopersicum
-
 cultivar Micro-Tom
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.267 leaf
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.267 eriodictyol + S-adenosyl-L-methionine 11% of the activity with quercetin Solanum lycopersicum homoeriodictyol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.267 laricitrin + S-adenosyl-L-methionine 114% of the activity with quercetin Solanum lycopersicum syringetin + S-adenosyl-L-homocysteine
-
 ?
2.1.1.267 luteolin + S-adenosyl-L-methionine 42% of the activity with quercetin Solanum lycopersicum chrysoeriol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.267 additional information enzyme is a regiospecific flavonoid 3'/5'-O-methyltransferase showing higher binding affinity and catalytic efficiency for quercetin and luteolin than for eriodictyol. No substrates: naringenin, apigenin, tricetin, kaempferol, daidzein, genistein Solanum lycopersicum ?
-
 ?
2.1.1.267 quercetin + S-adenosyl-L-methionine
-
 Solanum lycopersicum isorhamnetin + S-adenosyl-L-homocysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.267 OMT3
-
 Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.267 0.026
-
 quercetin pH 7.5, 30°C Solanum lycopersicum
2.1.1.267 0.03
-
 luteolin pH 7.5, 30°C Solanum lycopersicum
2.1.1.267 0.055
-
 eriodictyol pH 7.5, 30°C Solanum lycopersicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.267 2.45
-
 eriodictyol pH 7.5, 30°C Solanum lycopersicum
2.1.1.267 40.5
-
 luteolin pH 7.5, 30°C Solanum lycopersicum
2.1.1.267 45.6
-
 quercetin pH 7.5, 30°C Solanum lycopersicum