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Literature summary extracted from

  • Andreeva, N.; Trilisenko, L.; Kulakovskaya, T.; Dumina, M.; Eldarov, M.
    Purification and properties of recombinant exopolyphosphatase PPN1 and effects of its overexpression on polyphosphate in Saccharomyces cerevisiae (2015), J. Biosci. Bioeng., 119, 52-56.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.11 ammonium chloride 200 mM, stimulates almost twofold in the presence and absence of 0.1 mM Co2+ Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.11
-
 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.11 heparin
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.11 cytosol 42% of the activity in the lysate Saccharomyces cerevisiae 5829
-
3.6.1.11 vacuole 20% of the activity in the lysate Saccharomyces cerevisiae 5773
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.11 Co2+ best activator, maximum activation at 0.1 mM Saccharomyces cerevisiae
3.6.1.11 Mg2+ poor activation Saccharomyces cerevisiae
3.6.1.11 Mn2+ poor activition Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.11 33000
-
 x * 33000, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.11 Saccharomyces cerevisiae Q04119 bifunctional exo- and endopolyphosphatase, ECs 3.6.1.10 and 3.6.1.11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.11
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.11 (phosphate)15 + H2O 88% of the activity with (phosphate)25 Saccharomyces cerevisiae (phosphate)14 + phosphate
-
 ?
3.6.1.11 (phosphate)208 + H2O 100% activity Saccharomyces cerevisiae (phosphate)207 + phosphate
-
 ?
3.6.1.11 (phosphate)25 + H2O 100% activity Saccharomyces cerevisiae (phosphate)24 + phosphate
-
 ?
3.6.1.11 (phosphate)3 + H2O 14% of the activity with (phosphate)25 Saccharomyces cerevisiae (phosphate)2 + phosphate
-
 ?
3.6.1.11 (phosphate)45 + H2O 100% activity Saccharomyces cerevisiae (phosphate)44 + phosphate
-
 ?
3.6.1.11 (phosphate)9 + H2O 45% of the activity with (phosphate)25 Saccharomyces cerevisiae (phosphate)8 + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.11 ? x * 33000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.6.1.11 Ppn1
-
 Saccharomyces cerevisiae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6.1.11 additional information
-
 50% inhibition around 1 mg/L Saccharomyces cerevisiae heparin

General Information

EC Number General Information Comment Organism
3.6.1.11 physiological function strong constitutive overexpression of exopolyphosphatase PPN1 results in 28- and 11fold increase in activity compared to the PPN1 mutant and wild-type strains, respectively. The content of acid-soluble polyphosphate decreases about 6fold and the content of acid-insoluble polyphosphate decreases about 2.5fold in the cells of the transformant compared to the mutant strain Saccharomyces cerevisiae