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Literature summary extracted from

  • Cheng, C.; Chen, J.; Fang, C.; Xia, Y.; Shan, Y.; Liu, Y.; Wen, G.; Song, H.; Fang, W.
    Listeria monocytogenes aguA1, but not aguA2, encodes a functional agmatine deiminase: biochemical characterization of its catalytic properties and roles in acid tolerance (2013), J. Biol. Chem., 288, 26606-26615.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.12 expressed in Escherichia coli Rosetta (DE3) cells Listeria monocytogenes

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.12 C157G the mutant of inactive isoform AguA2 shows wild type enzyme activity Listeria monocytogenes
3.5.3.12 C356A inactive Listeria monocytogenes
3.5.3.12 D218A inactive Listeria monocytogenes
3.5.3.12 D218E inactive Listeria monocytogenes
3.5.3.12 D94A inactive Listeria monocytogenes
3.5.3.12 D94E inactive Listeria monocytogenes
3.5.3.12 E155A inactive Listeria monocytogenes
3.5.3.12 E155D inactive Listeria monocytogenes
3.5.3.12 G157C inactive Listeria monocytogenes
3.5.3.12 H216A inactive Listeria monocytogenes
3.5.3.12 H216R inactive Listeria monocytogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.12 Co2+
-
 Listeria monocytogenes
3.5.3.12 Cu2+
-
 Listeria monocytogenes
3.5.3.12 additional information no inhibitory effect on isoform AguA1 activity with Fe3+, Mg2+, and Ni2+ Listeria monocytogenes
3.5.3.12 Zn2+
-
 Listeria monocytogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.12 0.65
-
 agmatine at 25°C and pH 7.5 Listeria monocytogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.12 46800
-
 x * 46800, calculated from amino acid sequence Listeria monocytogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.12 agmatine + H2O Listeria monocytogenes
-
 N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O Listeria monocytogenes 10403S
-
 N-carbamoylputrescine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.12 Listeria monocytogenes
-
-
-
3.5.3.12 Listeria monocytogenes 10403S
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.12 nickel-chelated agarose gel column chromatography Listeria monocytogenes

Storage Stability

EC Number Storage Stability Organism
3.5.3.12 25°C, purified enzyme, 6 h, 80% loss of activity Listeria monocytogenes
3.5.3.12 4°C, purified enzyme, at least 1 month, 15% loss of activity Listeria monocytogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.12 agmatine + H2O
-
 Listeria monocytogenes N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 agmatine + H2O
-
 Listeria monocytogenes 10403S N-carbamoylputrescine + NH3
-
 ?
3.5.3.12 additional information L-arginine does not act as the substrate for isoform AguA1. The recombinant isoform AguA2 shows no deiminase activity Listeria monocytogenes ?
-
 ?
3.5.3.12 additional information L-arginine does not act as the substrate for isoform AguA1. The recombinant isoform AguA2 shows no deiminase activity Listeria monocytogenes 10403S ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.12 ? x * 46800, calculated from amino acid sequence Listeria monocytogenes

Synonyms

EC Number Synonyms Comment Organism
3.5.3.12 AgDI
-
 Listeria monocytogenes
3.5.3.12 AguA1
-
 Listeria monocytogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.12 25
-
-
 Listeria monocytogenes

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.3.12 5 35 about 65% activity at 5°C, about 90% activity at 15°C, 100% activity at 25°C, less than 40% activity at 35°C Listeria monocytogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.12 34.28
-
 agmatine at 25°C and pH 7.5 Listeria monocytogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.12 8.5
-
-
 Listeria monocytogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.12 3.5 10.5 more than 85% activity between pH 3.5 and 10.5 Listeria monocytogenes

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.3.12 0.034
-
 at 25°C and pH 7.5 Listeria monocytogenes Cu2+
3.5.3.12 0.25
-
 at 25°C and pH 7.5 Listeria monocytogenes Zn2+
3.5.3.12 2.89
-
 at 25°C and pH 7.5 Listeria monocytogenes Co2+

Expression

EC Number Organism Comment Expression
3.5.3.12 Listeria monocytogenes transcription is significantly induced at pH 5.0 up

General Information

EC Number General Information Comment Organism
3.5.3.12 malfunction deletion of aguA1 significantly impairs Listeria monocytogenes survival both in gastric fluid at pH 2.5 and in mouse stomach Listeria monocytogenes
3.5.3.12 physiological function the enzyme mediates acid tolerance in Listeria monocytogenes Listeria monocytogenes

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.3.12 53
-
 agmatine at 25°C and pH 7.5 Listeria monocytogenes