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Literature summary extracted from

  • Badarau, A.; Shi, Q.; Chow, J.W.; Zajicek, J.; Mobashery, S.; Vakulenko, S.
    Aminoglycoside 2''-phosphotransferase type IIIa from Enterococcus (2008), J. Biol. Chem., 283, 7638-7647.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.190 apramycin
-
 Enterococcus sp.
2.7.1.190 butirosin
-
 Enterococcus sp.
2.7.1.190 lividomycin A
-
 Enterococcus sp.
2.7.1.190 neamine
-
 Enterococcus sp.
2.7.1.190 neomycin
-
 Enterococcus sp.
2.7.1.190 paromomycin
-
 Enterococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.190 0.0045
-
 kanamycin A pH 7.5, 25°C Enterococcus sp.
2.7.1.190 0.0045
-
 GTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
2.7.1.190 0.039
-
 ITP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
2.7.1.190 0.09
-
 ATP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
2.7.1.190 0.1
-
 UTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.190 Enterococcus sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.190 ATP + kanamycin A
-
 Enterococcus sp. ADP + kanamycin A 2''-phosphate
-
 ?
2.7.1.190 GTP + gentamicin C
-
 Enterococcus sp. GDP + gentamicin C 2''-phosphate GTP is preferred over ATP ?
2.7.1.190 GTP + netilmicin
-
 Enterococcus sp. GDP + netilmicin 2''-phosphate
-
 ?
2.7.1.190 ITP + dibekacin
-
 Enterococcus sp. IDP + dibekacin 2''-phosphate
-
 ?
2.7.1.190 additional information random sequential Bi Bi mechanism. At pH 7.5 the release of guanosine triphosphate is rate-limiting. No substrates: amikacin, isepamicin, neomycin, butirosin, lividomycin A , paromomycin, spectinomycin, streptomycin, apramycin, hygromycin, neamine Enterococcus sp. ?
-
 ?
2.7.1.190 UTP + tobramycin
-
 Enterococcus sp. UDP + tobramycin 2''-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.190 aminoglycoside 2''-phosphotransferase type IIIa
-
 Enterococcus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.190 0.09
-
 ATP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
2.7.1.190 0.14
-
 UTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
2.7.1.190 0.5
-
 gentamicin C pH 7.5, 25°C Enterococcus sp.
2.7.1.190 0.7
-
 tobramycin pH 7.5, 25°C Enterococcus sp.
2.7.1.190 0.7
-
 netilmicin pH 7.5, 25°C Enterococcus sp.
2.7.1.190 0.7
-
 dibekacin pH 7.5, 25°C Enterococcus sp.
2.7.1.190 0.8
-
 kanamycin A pH 7.5, 25°C Enterococcus sp.
2.7.1.190 0.8
-
 GTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
2.7.1.190 2.1
-
 ITP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.190 1
-
 ATP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
2.7.1.190 1.4
-
 UTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
2.7.1.190 56
-
 ITP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
2.7.1.190 180
-
 kanamycin A pH 7.5, 25°C Enterococcus sp.
2.7.1.190 180
-
 GTP pH 7.5, 25°C, cosubstrate kanamycin A Enterococcus sp.
2.7.1.190 670
-
 netilmicin pH 7.5, 25°C Enterococcus sp.
2.7.1.190 1800
-
 gentamicin C pH 7.5, 25°C Enterococcus sp.
2.7.1.190 2200
-
 tobramycin pH 7.5, 25°C Enterococcus sp.
2.7.1.190 2300
-
 dibekacin pH 7.5, 25°C Enterococcus sp.