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Literature summary extracted from

  • Tran, A.X.; Lester, M.E.; Stead, C.M.; Raetz, C.R.; Maskell, D.J.; McGrath, S.C.; Cotter, R.J.; Trent, M.S.
    Resistance to the antimicrobial peptide polymyxin requires myristoylation of Escherichia coli and Salmonella typhimurium lipid A (2005), J. Biol. Chem., 280, 28186-28194.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.43 additional information generation of polymyxin-sensitive mutants of an Escherichia coli pmrAC strain WD101 producing a lipid A that lacked both the 3'-acyloxyacyl-linked myristate (C14) and L-4-aminoarabinose, even though the necessary enzymatic machinery required to synthesize L-4-aminoarabinose-modified lipid A is present. Strain WD103 is generated by P1vir transduction of the polymyxin-resistant genotype (pmrAC) of strain WD101 into the Escherichia coli lpxM mutant MLK1067, phenotype, overview. The polymyxin-sensitive parent strains MLK1067 and W3110 produce unmodified penta- or hexa-acylated lipid A, respectively. Complementation of WD103 with the vector pWSLpxM resulted in restoration of polymyxin resistance to the levels displayed by WD101, effect of lipid myristoylation on the polymyxin resistance Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.43 Escherichia coli
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 W3110, gene PmrA
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2.7.8.43 Salmonella enterica subsp. enterica serovar Typhimurium
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 a lpxM mutant strain, gene pmrA
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2.7.8.43 Salmonella enterica subsp. enterica serovar Typhimurium C5
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 a lpxM mutant strain, gene pmrA
-

Synonyms

EC Number Synonyms Comment Organism
2.7.8.43 PmrA
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.8.43 PmrA
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.7.8.43 malfunction in N-minimal media under Mg2+-limiting conditions to activate the PhoP/PhoQ two-component regulatory system, Salmonella typhimurium PhoP induces activation of PmrA, leading to increased substitution of lipid A phosphate groups with L-4-aminoarabinose and phosphoethanolamine. The mutant strain produces a lipopolysaccharide with an approximate 10fold decrease in the amount of myristate. The lipid myristoylation has an effect on the polymyxin resistance Salmonella enterica subsp. enterica serovar Typhimurium
2.7.8.43 malfunction WD101, a polymyxin-resistant Escherichia coli K-12 strain, contains a mutation in the pmrA (basR) gene resulting in a pmrAC phenotype promoting polymyxin resistance Escherichia coli
2.7.8.43 metabolism the polymyxin-resistant phenotype is primarily under the control of the PmrA/PmrB two-component regulatory system that is activated during growth under conditions of low pH, high Fe3+, and in a PhoP/PhoQ-dependent manner during Mg2+ starvation Escherichia coli
2.7.8.43 physiological function the enzyme regulates the modification of phosphate moieties of lipid A, which may be substituted with L-4-aminoarabinose or phosphoethanolamine groups Salmonella enterica subsp. enterica serovar Typhimurium
2.7.8.43 physiological function the enzyme regulates the modification of phosphate moieties of lipid A, which may be substituted with L-4-aminoarabinose or phosphoethanolamine groups. The enzyme also regulates the catalysis of periplasmic addition of L-4-aminoarabinose to lipid A through glycosyltransferase L-4-aminoarabinose transferase (ArnT) Escherichia coli