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Literature summary extracted from

  • Ertesvag, H.; Hoidal, H.K.; Skjak-Braek, G.; Valla, S.
    The Azotobacter vinelandii mannuronan C-5-epimerase AlgE1 consists of two separate catalytic domains (1998), J. Biol. Chem., 273, 30927-30932.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.37 expression in Escherichia coli Azotobacter vinelandii

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.37 additional information division of algE1 into two parts based on the modular type of structure, and expression of each part in Escherichia coli. AlgE1 contains two catalytic domains, AlgE1-1, which introduces both G-blocks and MG-blocks, and AlgE1-2, which only introduces MG-blocks. AlgE1-1 has a much lower specific activity than both AlgE1-2 and AlgE1 Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.37 Ca2+ required, optimum concentration 0.8 mM. The Ca2+ requirement of domain AlgE1-1 is much higher (optimum around 3 mM) than that of whole AlgE1, while domain AlgE1-2 needs slightly more Ca2+ than AlgE1 for optimal activity Azotobacter vinelandii
5.1.3.37 Mg2+ at Ca2+ concentrations lower than optimum, the addition of Sr2+ or Mg2+ has a stimulatory effect, but Mg2+ is not able to substitute for Ca2+ when no calcium is added Azotobacter vinelandii
5.1.3.37 Sr2+ may partly substitute for Ca2+. At Ca2+ concentrations lower than optimum, the addition of Sr2+ or Mg21 has a stimulatory effect Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.37 Azotobacter vinelandii Q44494
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.37 0.37
-
 catalytic domain AlgE1-1, pH 6.9, 37°C Azotobacter vinelandii
5.1.3.37 4.4
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 catalytic domain AlgE1-2, pH 6.9, 37°C Azotobacter vinelandii
5.1.3.37 4.9
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 pH 6.9, 37°C Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.37 [alginate]-beta-D-mannuronate
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 Azotobacter vinelandii [alginate]-alpha-L-guluronate reaction product of AlgE1 is a mixture of blocks of continuous G residues (G-blocks) and blocks containing alternating M and G residues (MG-blocks) ?