Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Verma, S.; Mahadevan, S.
    The ChbG gene of the chitobiose (chb) operon of Escherichia coli encodes a chitooligosaccharide deacetylase (2012), J. Bacteriol., 194, 4959-4971.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.105 gene chbG or ydjC, encoded in the chb operon, DNA and amino acid sequence determination and analysis Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.105 F146L site-directed mutagenesis Escherichia coli
3.5.1.105 K184E site-directed mutagenesis Escherichia coli
3.5.1.105 additional information growth of different mutant strains on various beta-glucosides as the sole carbon source. Effect of chbG deletion on transcriptional activation of the chb promoter in Cel+ mutants carrying a deletion of nagC and different activating mutations in chbR, overview. The Vibrio cholerae homologue of chbG can rescue the effect of the Escherichia coli chbG mutation Escherichia coli
3.5.1.105 N137K/K184E site-directed mutagenesis Escherichia coli
3.5.1.105 N238S site-directed mutagenesis Escherichia coli
3.5.1.105 S134P site-directed mutagenesis Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.105 GlcNAc-beta-(1,4)-GlcNAc + H2O Escherichia coli
-
 GlcNAc-beta-(1,4)-GlcN + acetate
-
 ?
3.5.1.105 GlcNAc-beta-(1,4)-GlcNAc + H2O Escherichia coli BW25113
-
 GlcNAc-beta-(1,4)-GlcN + acetate
-
 ?
3.5.1.105 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O Escherichia coli
-
 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcN + acetate
-
 ?
3.5.1.105 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O Escherichia coli BW25113
-
 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcN + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.105 Escherichia coli P37794 gene chbG or ydjC; gene chbG or ydjC, encoded in the chb operon
-
3.5.1.105 Escherichia coli BW25113 P37794 gene chbG or ydjC; gene chbG or ydjC, encoded in the chb operon
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.105 GlcNAc-beta-(1,4)-GlcNAc + H2O
-
 Escherichia coli GlcNAc-beta-(1,4)-GlcN + acetate
-
 ?
3.5.1.105 GlcNAc-beta-(1,4)-GlcNAc + H2O
-
 Escherichia coli BW25113 GlcNAc-beta-(1,4)-GlcN + acetate
-
 ?
3.5.1.105 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O
-
 Escherichia coli GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcN + acetate
-
 ?
3.5.1.105 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O
-
 Escherichia coli BW25113 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcN + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.105 ChbG
-
 Escherichia coli
3.5.1.105 chitooligosaccharide deacetylase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.105 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.105 7
-
 assay at Escherichia coli

General Information

EC Number General Information Comment Organism
3.5.1.105 evolution the enzyme is an evolutionarily conserved protein that belongs to the the YdjC family of proteins Escherichia coli
3.5.1.105 metabolism deacetylated chitobiose and chitotriose provide the induction signal necessary for the activation of the ChbR regulator Escherichia coli
3.5.1.105 additional information three conserved residues Asp11, His61, and His125 form the catalytic triad Escherichia coli
3.5.1.105 physiological function the monodeacetylase that is essential for growth on the acetylated chitooligosaccharides chitobiose and chitotriose but is dispensable for growth on cellobiose and chitosan dimer, the deacetylated form of chitobiose. Activation of the chb promoter by the regulatory protein ChbR is dependent on ChbG, deacetylation of chitobiose-6-phosphate and chitotriose-6-phosphate is necessary for their recognition by ChbR as inducers. ChbR-independent expression of the permease and phospho-beta-glucosidase from a heterologous promoter did not support growth on both chitobiose and chitotriose in the absence of chbG, suggesting an additional role of chbG in the hydrolysis of chitooligosaccharides Escherichia coli