EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.12.23 | additional information | construction of hybrid dioxygenases with the genes encoding 2-nitrotoluene 2,3-dioxygenase from Pseudomonas sp. strain JS42 and 2,4-dinitrotoluene dioxygenase from Burkholderia sp. strain DNT. The C-terminal region of the large subunit of the oxygenase component is responsible for the enzyme specificity differences observed between 2-nitrotoluene 2,3-dioxygenase and 2,4-dinitrotoluene dioxygenase | Pseudomonas sp. |
1.14.12.24 | additional information | construction of hybrid dioxygenases with the genes encoding 2-nitrotoluene 2,3-dioxygenase from Pseudomonas sp. strain JS42 and 2,4-dinitrotoluene dioxygenase from Burkholderia sp. strain DNT. The C-terminal region of the large subunit of the oxygenase component is responsible for the enzyme specificity differences observed between 2-nitrotoluene 2,3-dioxygenase and 2,4-dinitrotoluene dioxygenase | Burkholderia sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.23 | Pseudomonas sp. | - |
- |
- |
1.14.12.24 | Burkholderia sp. | - |
- |
- |
1.14.12.24 | Burkholderia sp. DNT | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.23 | 2-nitrotoluene + NADH + O2 | - |
Pseudomonas sp. | 3-methylcatechol + nitrite + NAD+ | formation of 10 nmol 3-methylcatechol per mg protein plus 1.06 nmol 2-nitrobenzyl alcohol | ? | |
1.14.12.23 | 3-nitrotoluene + NADH + O2 | - |
Pseudomonas sp. | 4-methylcatechol + nitrite + NAD+ | formation of 0.06 nmol 4-methylcatechol per mg protein plus 0.03 nmol 3-nitrobenzyl alcohol | ? | |
1.14.12.23 | additional information | no substrate: 2,4-dinitrotoluene | Pseudomonas sp. | ? | - |
? | |
1.14.12.23 | naphthalene + O2 | - |
Pseudomonas sp. | cis-1,2-dihydroxy-1,2-dihydronaphthalene | formation of 0.75 nmol 3-methylcatechol per mg protein | ? | |
1.14.12.24 | 2,4-dinitrotoluene + NADH + O2 | - |
Burkholderia sp. | 4-methyl-5-nitrocatechol + nitrite + NAD+ | formation of 9.54 nmol 3-methylcatechol per mg protein plus 1.06 nmol 2-nitrobenzyl alcohol | ? | |
1.14.12.24 | 2,4-dinitrotoluene + NADH + O2 | - |
Burkholderia sp. DNT | 4-methyl-5-nitrocatechol + nitrite + NAD+ | formation of 9.54 nmol 3-methylcatechol per mg protein plus 1.06 nmol 2-nitrobenzyl alcohol | ? | |
1.14.12.24 | 3-nitrotoluene + NADH + O2 | - |
Burkholderia sp. | 4-methylcatechol + nitrite + NAD+ | formation of 0.06 nmol 4-methylcatechol per mg protein plus 0.03 nmol 3-nitrobenzyl alcohol | ? | |
1.14.12.24 | 3-nitrotoluene + NADH + O2 | - |
Burkholderia sp. DNT | 4-methylcatechol + nitrite + NAD+ | formation of 0.06 nmol 4-methylcatechol per mg protein plus 0.03 nmol 3-nitrobenzyl alcohol | ? | |
1.14.12.24 | additional information | no substrate: 2-nitrotoluene, 3-nitrotoluene | Burkholderia sp. | ? | - |
? | |
1.14.12.24 | additional information | no substrate: 2-nitrotoluene, 3-nitrotoluene | Burkholderia sp. DNT | ? | - |
? | |
1.14.12.24 | naphthalene + O2 | - |
Burkholderia sp. | cis-1,2-dihydroxy-1,2-dihydronaphthalene | formation of 0.83 nmol 3-methylcatechol per mg protein | ? | |
1.14.12.24 | naphthalene + O2 | - |
Burkholderia sp. DNT | cis-1,2-dihydroxy-1,2-dihydronaphthalene | formation of 0.83 nmol 3-methylcatechol per mg protein | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.12.23 | 2NTDO | - |
Pseudomonas sp. |
1.14.12.24 | 2,4-dinitrotoluene dioxygenase | - |
Burkholderia sp. |
1.14.12.24 | 2,4-DNTDO | - |
Burkholderia sp. |