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Literature summary extracted from

  • Silakov, A.; Grove, T.L.; Radle, M.I.; Bauerle, M.R.; Green, M.T.; Rosenzweig, A.C.; Boal, A.K.; Booker, S.J.
    Characterization of a cross-linked protein-nucleic acid substrate radical in the reaction catalyzed by RlmN (2014), J. Am. Chem. Soc., 136, 8221-8228.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.192 mutant C118A, hanging drop vapor diffusion method, using 7.5% (w/v) PEG 6000, 0.1 M HEPES, pH 7.5, and 5% (v/v) 2-methyl-2,4-pentanediol as the precipitant Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.192 C118A inactive Escherichia coli
2.1.1.192 C118S inactive Escherichia coli
2.1.1.192 E105A the mutant shows wild type activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.192 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Escherichia coli the enzyme only catalyzes C2 methylation of adenine2503 in 23S rRNA S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.192 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.192 S-adenosyl-L-methionine + adenine2503 in 23S rRNA the enzyme only catalyzes C2 methylation of adenine2503 in 23S rRNA Escherichia coli S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.192 RlmN
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.192 [4Fe-4S]-center
-
 Escherichia coli