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Literature summary extracted from

  • Kasai, K.; Nishiyama, N.; Yamauchi, K.
    Characterization of Oncorhynchus mykiss 5-hydroxyisourate hydrolase/transthyretin superfamily: evolutionary and functional analyses (2013), Gene, 531, 326-336.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.17 phylogenetic analysis, sequence comparisons, quantitative real-time PCR enzyme expression analysis, recombinant expression of soluble His-tagged enzyme in Escherichia coli Oncorhynchus mykiss

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.17 Zn2+ significant inhibition at 0.05 mM Oncorhynchus mykiss

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.17 additional information the enzyme is not affected by 1 mM of Cr3+, Mg2+, or Ca2+ Oncorhynchus mykiss

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.17 14000
-
4 * 14000, SDS-PAGE Oncorhynchus mykiss
3.5.2.17 40000
-
gel filtration Oncorhynchus mykiss

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.17 5-hydroxyisourate + H2O Oncorhynchus mykiss
-
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.17 Oncorhynchus mykiss U6C7K1 HIUHase 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.17 recombinant soluble His-tagged enzyme from Escherichia coli by metal affinity chromatography Oncorhynchus mykiss

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.17 brain
-
Oncorhynchus mykiss
-
3.5.2.17 eye
-
Oncorhynchus mykiss
-
3.5.2.17 gill
-
Oncorhynchus mykiss
-
3.5.2.17 heart
-
Oncorhynchus mykiss
-
3.5.2.17 intestine
-
Oncorhynchus mykiss
-
3.5.2.17 kidney
-
Oncorhynchus mykiss
-
3.5.2.17 liver highest enzyme level Oncorhynchus mykiss
-
3.5.2.17 additional information tissue distribution pattern Oncorhynchus mykiss
-
3.5.2.17 stomach
-
Oncorhynchus mykiss
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.17 5-hydroxyisourate + H2O
-
Oncorhynchus mykiss 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
?

Subunits

EC Number Subunits Comment Organism
3.5.2.17 homotetramer 4 * 14000, SDS-PAGE Oncorhynchus mykiss

Synonyms

EC Number Synonyms Comment Organism
3.5.2.17 5-hydroxyisourate hydrolase
-
Oncorhynchus mykiss
3.5.2.17 HIUHase
-
Oncorhynchus mykiss
3.5.2.17 HIUHase 1
-
Oncorhynchus mykiss

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.17 25 27 assay at Oncorhynchus mykiss

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.17 7.5
-
assay at Oncorhynchus mykiss

General Information

EC Number General Information Comment Organism
3.5.2.17 evolution the enzyme belongs to the 5-hydroxyisourate hydrolase/transthyretin superfamily: evolutionary and functional analyses, overview. Teleosts have highly diverged genomes that resulted from whole genome duplication, which leads to an extensive diversity of paralogous genes. Transthyretin, an extracellular thyroid hormone binding protein, is thought to have evolved from an ancestral 5-hydroxyisourate hydrolase by gene duplication at some stage of chordate evolution. Phylogenetic analysis of the teleost aa sequences reveals the presence of two HIUHase subfamilies, HIUHase 1 (which has an N-terminal peroxisomal targeting signal-2) and HIUHase 2 (which does not have an N-terminal PTS2), and one transthyretin family Oncorhynchus mykiss
3.5.2.17 metabolism the enzyme catalyzes the hydrolysis of 5-hydroxyisourate in the purine degradation pathway, and transthyretin, a thyroid hormone binding protein Oncorhynchus mykiss