EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.2.17 | phylogenetic analysis, sequence comparisons, quantitative real-time PCR enzyme expression analysis, recombinant expression of soluble His-tagged enzyme in Escherichia coli | Oncorhynchus mykiss |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.2.17 | Zn2+ | significant inhibition at 0.05 mM | Oncorhynchus mykiss |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.2.17 | additional information | the enzyme is not affected by 1 mM of Cr3+, Mg2+, or Ca2+ | Oncorhynchus mykiss |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.2.17 | 14000 | - |
4 * 14000, SDS-PAGE | Oncorhynchus mykiss |
3.5.2.17 | 40000 | - |
gel filtration | Oncorhynchus mykiss |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.2.17 | 5-hydroxyisourate + H2O | Oncorhynchus mykiss | - |
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.2.17 | Oncorhynchus mykiss | U6C7K1 | HIUHase 1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.2.17 | recombinant soluble His-tagged enzyme from Escherichia coli by metal affinity chromatography | Oncorhynchus mykiss |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.2.17 | brain | - |
Oncorhynchus mykiss | - |
3.5.2.17 | eye | - |
Oncorhynchus mykiss | - |
3.5.2.17 | gill | - |
Oncorhynchus mykiss | - |
3.5.2.17 | heart | - |
Oncorhynchus mykiss | - |
3.5.2.17 | intestine | - |
Oncorhynchus mykiss | - |
3.5.2.17 | kidney | - |
Oncorhynchus mykiss | - |
3.5.2.17 | liver | highest enzyme level | Oncorhynchus mykiss | - |
3.5.2.17 | additional information | tissue distribution pattern | Oncorhynchus mykiss | - |
3.5.2.17 | stomach | - |
Oncorhynchus mykiss | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.2.17 | 5-hydroxyisourate + H2O | - |
Oncorhynchus mykiss | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.2.17 | homotetramer | 4 * 14000, SDS-PAGE | Oncorhynchus mykiss |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.2.17 | 5-hydroxyisourate hydrolase | - |
Oncorhynchus mykiss |
3.5.2.17 | HIUHase | - |
Oncorhynchus mykiss |
3.5.2.17 | HIUHase 1 | - |
Oncorhynchus mykiss |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.2.17 | 25 | 27 | assay at | Oncorhynchus mykiss |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.2.17 | 7.5 | - |
assay at | Oncorhynchus mykiss |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.2.17 | evolution | the enzyme belongs to the 5-hydroxyisourate hydrolase/transthyretin superfamily: evolutionary and functional analyses, overview. Teleosts have highly diverged genomes that resulted from whole genome duplication, which leads to an extensive diversity of paralogous genes. Transthyretin, an extracellular thyroid hormone binding protein, is thought to have evolved from an ancestral 5-hydroxyisourate hydrolase by gene duplication at some stage of chordate evolution. Phylogenetic analysis of the teleost aa sequences reveals the presence of two HIUHase subfamilies, HIUHase 1 (which has an N-terminal peroxisomal targeting signal-2) and HIUHase 2 (which does not have an N-terminal PTS2), and one transthyretin family | Oncorhynchus mykiss |
3.5.2.17 | metabolism | the enzyme catalyzes the hydrolysis of 5-hydroxyisourate in the purine degradation pathway, and transthyretin, a thyroid hormone binding protein | Oncorhynchus mykiss |