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Literature summary extracted from

  • Yusuf, F.; Rather, I.A.; Jamwal, U.; Gandhi, S.G.; Chaubey, A.
    Cloning and functional characterization of nitrilase from Fusarium proliferatum AUF-2 for detoxification of nitriles (2015), Funct. Integr. Genomics, 15, 413-424.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.5.7 EDTA about 130% activity at 1 mM Fusarium proliferatum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.7 expressed in Escherichia coli BL21 (DE3) cells Fusarium proliferatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.7 Ag+ complete inhibition at 1 mM Fusarium proliferatum
3.5.5.7 Ba2+ about 55% residual activity at 1 mM Fusarium proliferatum
3.5.5.7 Co2+ about 65% residual activity at 1 mM Fusarium proliferatum
3.5.5.7 Cu2+ about 75% residual activity at 1 mM Fusarium proliferatum
3.5.5.7 Dichloromethane about 30% residual activity at 5% (v/v) Fusarium proliferatum
3.5.5.7 ethanol about 55% residual activity at 5% (v/v) Fusarium proliferatum
3.5.5.7 hexane about 20% residual activity at 5% (v/v) Fusarium proliferatum
3.5.5.7 Li+ about 80% residual activity at 1 mM Fusarium proliferatum
3.5.5.7 methanol about 75% residual activity at 5% (v/v) Fusarium proliferatum
3.5.5.7 Ni2+ about 60% residual activity at 1 mM Fusarium proliferatum
3.5.5.7 Propanol about 22% residual activity at 5% (v/v) Fusarium proliferatum
3.5.5.7 Toluene about 5% residual activity at 5% (v/v) Fusarium proliferatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.7 1.55
-
 Benzonitrile at pH 8.0 and 37°C Fusarium proliferatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.5.7 Ca2+ about 115% activity at 1 mM Fusarium proliferatum
3.5.5.7 Fe2+ about 110% activity at 1 mM Fusarium proliferatum
3.5.5.7 Fe3+ about 125% activity at 1 mM Fusarium proliferatum
3.5.5.7 Mg2+ about 125% activity at 1 mM Fusarium proliferatum
3.5.5.7 Mn2+ about 120% activity at 1 mM Fusarium proliferatum
3.5.5.7 Zn2+ about 120% activity at 1 mM Fusarium proliferatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.7 37000
-
 x * 37000, SDS-PAGE Fusarium proliferatum

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.7 Fusarium proliferatum A0A0E3D8K7
-
-
3.5.5.7 Fusarium proliferatum AUF-2 A0A0E3D8K7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.7 Ni-NTA column chromatography Fusarium proliferatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.7 2-cyanopyridine + 2 H2O 110% activity compared to benzonitrile Fusarium proliferatum pyridine 2-carboxylic acid + NH3
-
 ?
3.5.5.7 3-aminobutyronitrile + H2O 201% activity compared to benzonitrile Fusarium proliferatum 3-aminobutyric acid + NH3
-
 ?
3.5.5.7 3-cyanopyridine + 2 H2O 151% activity compared to benzonitrile Fusarium proliferatum pyridine 3-carboxylic acid + NH3
-
 ?
3.5.5.7 3-nitrobenzonitrile + H2O 110% activity compared to benzonitrile Fusarium proliferatum 3-nitrobenzoic acid + NH3
-
 ?
3.5.5.7 3-phenylpropionitrile + H2O 151% activity compared to benzonitrile Fusarium proliferatum 3-phenylpropionic acid + NH3
-
 ?
3.5.5.7 3-phenylpropionitrile + H2O 151% activity compared to benzonitrile Fusarium proliferatum AUF-2 3-phenylpropionic acid + NH3
-
 ?
3.5.5.7 4-aminobutyronitrile + H2O 251% activity compared to benzonitrile Fusarium proliferatum 4-aminobutyric acid + NH3
-
 ?
3.5.5.7 4-cyanopyridine + 2 H2O 225% activity compared to benzonitrile Fusarium proliferatum pyridine 4-carboxylic acid + NH3
-
 ?
3.5.5.7 4-nitrobenzonitrile + H2O 151% activity compared to benzonitrile Fusarium proliferatum 4-nitrobenzoic acid + NH3
-
 ?
3.5.5.7 acetonitrile + H2O 135% activity compared to benzonitrile Fusarium proliferatum acetate + NH3
-
 ?
3.5.5.7 acetonitrile + H2O 135% activity compared to benzonitrile Fusarium proliferatum AUF-2 acetate + NH3
-
 ?
3.5.5.7 acrylonitrile + H2O 400% activity compared to benzonitrile Fusarium proliferatum acrylic acid + NH3
-
 ?
3.5.5.7 acrylonitrile + H2O 400% activity compared to benzonitrile Fusarium proliferatum AUF-2 acrylic acid + NH3
-
 ?
3.5.5.7 benzonitrile + 2 H2O 100% activity Fusarium proliferatum benzoate + NH3
-
 ?
3.5.5.7 butyronitrile + H2O 425% activity compared to benzonitrile Fusarium proliferatum butyrate + NH3
-
 ?
3.5.5.7 diphenylacetonitrile + H2O 72% activity compared to benzonitrile Fusarium proliferatum diphenylacetic acid + NH3
-
 ?
3.5.5.7 glutaronitrile + H2O 319% activity compared to benzonitrile Fusarium proliferatum glutaric acid + NH3
-
 ?
3.5.5.7 mandelonitrile + 2 H2O 69% activity compared to benzonitrile Fusarium proliferatum mandelic acid + NH3
-
 ?
3.5.5.7 mandelonitrile + 2 H2O 69% activity compared to benzonitrile Fusarium proliferatum AUF-2 mandelic acid + NH3
-
 ?
3.5.5.7 additional information no activity with fumaronitrile and succinonitrile Fusarium proliferatum ?
-
 ?
3.5.5.7 additional information no activity with fumaronitrile and succinonitrile Fusarium proliferatum AUF-2 ?
-
 ?
3.5.5.7 propionitrile + H2O 416% activity Fusarium proliferatum propionic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.7 ? x * 37000, SDS-PAGE Fusarium proliferatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.7 37
-
-
 Fusarium proliferatum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.5.7 25 45 more than 60% activity between 25 and 45°C Fusarium proliferatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.7 8
-
-
 Fusarium proliferatum