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Literature summary extracted from

  • Bae, J.; Kim, S.M.; Lee, S.B.
    Identification and characterization of 2-keto-3-deoxy-L-rhamnonate dehydrogenase belonging to the MDR superfamily from the thermoacidophilic bacterium Sulfobacillus thermosulfidooxidans: implications to L-rhamnose metabolism in archaea (2015), Extremophiles, 19, 469-478.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.401 expressed in Escherichia coli DH5alpha cells Sulfobacillus thermosulfidooxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.401 EDTA 5 mM, 73% residual activity; 73.7% residual activity at 5 mM EDTA Sulfobacillus thermosulfidooxidans
1.1.1.401 EGTA 5 mM, 72% residual activity; 72.9% residual activity at 5 mM EGTA Sulfobacillus thermosulfidooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.401 0.42
-
 NAD+ pH 9.5, 50°C Sulfobacillus thermosulfidooxidans
1.1.1.401 0.42
-
 NAD+ at pH 9.5 and 50°C Sulfobacillus thermosulfidooxidans
1.1.1.401 2
-
 2-dehydro-3-deoxy-L-rhamnonate pH 9.5, 50°C Sulfobacillus thermosulfidooxidans
1.1.1.401 2
-
 2-dehydro-3-deoxy-L-rhamnonate at pH 9.5 and 50°C Sulfobacillus thermosulfidooxidans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.401 Co2+ 5 mM, 118% of initial activity Sulfobacillus thermosulfidooxidans
1.1.1.401 Co2+ 118.6% activity at 5 mM Sulfobacillus thermosulfidooxidans
1.1.1.401 additional information activity is not significantly affected by the addition of 5 mM metal ions Sulfobacillus thermosulfidooxidans
1.1.1.401 additional information the enzyme activity is not significantly affected by the addition of 5 mM Ca2+, Zn2+, Mg2+, Mn2+, Fe2+, Fe3+, and Ni2+ Sulfobacillus thermosulfidooxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.401 2-dehydro-3-deoxy-L-rhamnonate + NAD+ Sulfobacillus thermosulfidooxidans
-
 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?
1.1.1.401 2-dehydro-3-deoxy-L-rhamnonate + NAD+ Sulfobacillus thermosulfidooxidans AT-1
-
 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.173 Sulfobacillus thermosulfidooxidans
-
-
-
1.1.1.401 Sulfobacillus thermosulfidooxidans
-
-
-
1.1.1.401 Sulfobacillus thermosulfidooxidans P0DOW0
-
-
1.1.1.401 Sulfobacillus thermosulfidooxidans AT-1 P0DOW0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.401 Ni-NTA column chromatography and Q-Sepharose column chromatography Sulfobacillus thermosulfidooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.173 1.11
-
 substrate L-rhamnose, cosubstrate NADP+, pH 9.5, 50°C Sulfobacillus thermosulfidooxidans
1.1.1.173 1.22
-
 substrate L-mannose, cosubstrate NAD+, pH 9.5, 50°C Sulfobacillus thermosulfidooxidans
1.1.1.173 22
-
 substrate L-lyxose, cosubstrate NAD+, pH 9.5, 50°C Sulfobacillus thermosulfidooxidans
1.1.1.173 33
-
 substrate L-rhamnose, cosubstrate NAD+, pH 9.5, 50°C Sulfobacillus thermosulfidooxidans
1.1.1.401 0.78
-
 unpurified enzyme, at pH 9.0 and 50°C Sulfobacillus thermosulfidooxidans
1.1.1.401 4.06
-
 pH 9.5, 50°C Sulfobacillus thermosulfidooxidans
1.1.1.401 4.06
-
 after 5.1fold purification, at pH 9.0 and 50°C Sulfobacillus thermosulfidooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.173 L-lyxose + NAD+
-
 Sulfobacillus thermosulfidooxidans ? + NADH
-
 ?
1.1.1.173 L-mannose + NAD+
-
 Sulfobacillus thermosulfidooxidans ? + NADH
-
 ?
1.1.1.173 L-rhamnose + NAD+
-
 Sulfobacillus thermosulfidooxidans L-rhamno-1,4-lactone + NADH
-
 ?
1.1.1.173 L-rhamnose + NADP+
-
 Sulfobacillus thermosulfidooxidans L-rhamno-1,4-lactone + NADPH
-
 ?
1.1.1.173 additional information no substrates are: D-glucose, D-mannose, D-galactose, D-fructose, D-xylose, D-fucose, D-arabinose, D-lyxose, D-ribose, D-fucose, L-fucose, L-xylose, L-arabinose, L-sorbose, sorbitol, mannitol, xylitol, 2,3-butanediol, 2-dehydro-3-deoxy-L-rhamnonate, and L-rhamnonate Sulfobacillus thermosulfidooxidans ?
-
 ?
1.1.1.401 2,3-butanediol + NAD+ the enzyme also shows weak 2,3-butanediol dehydrogenase activity (11.3% compared to 2-dehydro-3-deoxy-L-rhamnonate) Sulfobacillus thermosulfidooxidans ?
-
 ?
1.1.1.401 2,3-butanediol + NAD+ the enzyme also shows weak 2,3-butanediol dehydrogenase activity (11.3% compared to 2-dehydro-3-deoxy-L-rhamnonate) Sulfobacillus thermosulfidooxidans AT-1 ?
-
 ?
1.1.1.401 2-dehydro-3-deoxy-L-rhamnonate + NAD+
-
 Sulfobacillus thermosulfidooxidans 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?
1.1.1.401 2-dehydro-3-deoxy-L-rhamnonate + NAD+ 100% activity Sulfobacillus thermosulfidooxidans 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?
1.1.1.401 2-dehydro-3-deoxy-L-rhamnonate + NAD+
-
 Sulfobacillus thermosulfidooxidans AT-1 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?
1.1.1.401 2-dehydro-3-deoxy-L-rhamnonate + NAD+ 100% activity Sulfobacillus thermosulfidooxidans AT-1 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+
-
 ?
1.1.1.401 additional information enzyme shows low activity towards 2,3-butanediol (11% of the activity with 2-dehydro-3-deoxy-L-rhamnonate), and very little activity towards L-rhamnonate, 2-dehydro-3-deoxy-L-mannonate, L-lyxonate and L-mannonate. No substrates are: aldoses such as D-glucose, D-mannose, D-galactose, D-fructose, D-xylose, D-fucose, D-arabinose, D-lyxose, D-ribose, D-fucose, L-fucose, L-xylose, L-arabinose, L-sorbose, polyols such as sorbitol, mannitol, xylitol, amino acids such as L-threonine, L-serine, alcohols such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 1,2-propandiol, 1,3-propandiol, aldonates such as D-gluconate, D-galactonate, D-mannonate, D-arabinonate, D-lyxonate, D-xylonate, organic acids such as pyruvate, D-lactate, L-lactate, aldehydes such as D-lactaldehyde, L-lactaldehyde, acetaldehyde, DL-glyceraldehyde, glycolaldehyde Sulfobacillus thermosulfidooxidans ?
-
 ?
1.1.1.401 additional information the enzyme shows less than 5% activity with L-rhamnonate, 2-dehydro-3-deoxy-L-mannonate, L-lyxonate, and L-mannonate. The enzyme does not show any detectable activity in the presence of NADP+ Sulfobacillus thermosulfidooxidans ?
-
 ?
1.1.1.401 additional information the enzyme shows less than 5% activity with L-rhamnonate, 2-dehydro-3-deoxy-L-mannonate, L-lyxonate, and L-mannonate. The enzyme does not show any detectable activity in the presence of NADP+ Sulfobacillus thermosulfidooxidans AT-1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.173 Sulth_3559
-
 Sulfobacillus thermosulfidooxidans
1.1.1.401 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase
-
 Sulfobacillus thermosulfidooxidans
1.1.1.401 2-keto-3-deoxy-L-rhamnonate dehydrogenase
-
 Sulfobacillus thermosulfidooxidans
1.1.1.401 KDRDH
-
 Sulfobacillus thermosulfidooxidans
1.1.1.401 L-KDR dehydrogenase
-
 Sulfobacillus thermosulfidooxidans
1.1.1.401 Sulth 3557 protein
-
 Sulfobacillus thermosulfidooxidans
1.1.1.401 Sulth_3557
-
 Sulfobacillus thermosulfidooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.173 60
-
-
 Sulfobacillus thermosulfidooxidans
1.1.1.401 50
-
-
 Sulfobacillus thermosulfidooxidans
1.1.1.401 60
-
-
 Sulfobacillus thermosulfidooxidans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.401 40 80 after 20 min incubation, the enzyme is stable at temperatures below 60°C, maintains 53.8% activity at 70°C, and is almost completely inactive at 80°C Sulfobacillus thermosulfidooxidans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.401 60
-
 20 min, stable Sulfobacillus thermosulfidooxidans
1.1.1.401 70
-
 20 min, 53% residual activity Sulfobacillus thermosulfidooxidans
1.1.1.401 80
-
 20 min, inactive Sulfobacillus thermosulfidooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.173 9.5
-
-
 Sulfobacillus thermosulfidooxidans
1.1.1.401 9.5
-
-
 Sulfobacillus thermosulfidooxidans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.401 7 11.5 little or no activity below pH 7.0, at pH 11.5 the enzme shows 75% of the activity at pH 9.5 Sulfobacillus thermosulfidooxidans
1.1.1.401 8 11 the enzyme has no or little activity at pH below 7.0, about 50% activity at pH 8.0, while the enzyme activity at pH 11.0 is about 75% of that obtained at pH 9.5 Sulfobacillus thermosulfidooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.173 NAD+ NAD+ is preferred over NADP+ Sulfobacillus thermosulfidooxidans
1.1.1.173 NADP+ about 30fold less activity than with NAD+ Sulfobacillus thermosulfidooxidans
1.1.1.401 additional information no detectable activity with NADP+ Sulfobacillus thermosulfidooxidans
1.1.1.401 NAD+
-
 Sulfobacillus thermosulfidooxidans