EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.167 | (R)-2-hydroxyglutaryl-CoA dehydratase component A | - |
[Clostridium] symbiosum | |
4.2.1.167 | FMNH2 | 1 mol per mol heterodimeric enzyme | [Clostridium] symbiosum | |
4.2.1.167 | riboflavin | 0.3 mol per mol heterodimeric enzyme | [Clostridium] symbiosum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.167 | - |
[Clostridium] symbiosum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.167 | 4Fe-4S centre | the enzyme contains two [4Fe4S]2+ clusters. It is likely that the alpha and beta subunits are bridged by a [4Fe4S]2+ cluster. The enzyme contains 7.5 non-heme iron and 8.1 acid labile sulfur/heterodimer, symmetric cube-type structures | [Clostridium] symbiosum | |
4.2.1.167 | [4Fe-4S]2+ cluster | 7.3 mol iron per mol heterodimeric enzyme, 8.1 mol sulfur per mol heterodimeric enzyme | [Clostridium] symbiosum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.167 | 42000 | - |
- |
[Clostridium] symbiosum |
4.2.1.167 | 42000 | - |
1 * 54000, 1 * 42000, SDS-PAGE | [Clostridium] symbiosum |
4.2.1.167 | 54000 | - |
- |
[Clostridium] symbiosum |
4.2.1.167 | 54000 | - |
1 * 54000, 1 * 42000, SDS-PAGE | [Clostridium] symbiosum |
4.2.1.167 | 100000 | - |
gel filtration | [Clostridium] symbiosum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.167 | [Clostridium] symbiosum | Q9X5B7 and Q9X5B8 | - |
- |
4.2.1.167 | [Clostridium] symbiosum | Q9X5B8 AND Q9X5B7 | Q9X5B8: subunit beta (hgdB), Q9X5B7: subunit alpha (hgdA) | - |
4.2.1.167 | [Clostridium] symbiosum DSM 934 | Q9X5B7 and Q9X5B8 | - |
- |
4.2.1.167 | [Clostridium] symbiosum HB 25 | Q9X5B8 AND Q9X5B7 | Q9X5B8: subunit beta (hgdB), Q9X5B7: subunit alpha (hgdA) | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
4.2.1.167 | oxygen sensitive with a half-life of 2 days under aerobic conditions | [Clostridium] symbiosum |
4.2.1.167 | the enzyme is oxygen sensitive with a half-life of 2 days under aerobic conditions | [Clostridium] symbiosum |
4.2.1.167 | under anaerobic conditions at 4°C for about 1 week without loss of activity | [Clostridium] symbiosum |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.167 | - |
[Clostridium] symbiosum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.167 | 32 | - |
strictly anaerobic conditions, substrate (R)-2-hydroxyglutaryl-CoA, 200 mM potassium phosphate pH 7.0, 22°C | [Clostridium] symbiosum |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.167 | -80°C, frozen with liquid nitrogen, stable | [Clostridium] symbiosum |
4.2.1.167 | -80°C, liquid nitrogen, long-term storage | [Clostridium] symbiosum |
4.2.1.167 | 4°C anaerobic conditions, 1 week stable | [Clostridium] symbiosum |
4.2.1.167 | 4°C, 1 week, anaerobic conditions, stable | [Clostridium] symbiosum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.167 | (R)-2-hydroxyglutaryl-CoA | - |
[Clostridium] symbiosum | (E)-glutaconyl-CoA + H2O | - |
r | |
4.2.1.167 | (R)-2-hydroxyglutaryl-CoA | - |
[Clostridium] symbiosum DSM 934 | (E)-glutaconyl-CoA + H2O | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.167 | heterodimer | 1 * 54000, 1 * 42000, SDS-PAGE | [Clostridium] symbiosum |
4.2.1.167 | heterodimer | 1 * 54000 (alpha) + 1 * 42000 (beta), SDS-PAGE | [Clostridium] symbiosum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.167 | hgdAB | - |
[Clostridium] symbiosum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.167 | riboflavin 5'-phosphate | the enzyme contains 1.0 mol of riboflavin 5'-phosphate per mol of heterodimeric enzyme | [Clostridium] symbiosum |