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Literature summary extracted from

  • Steigedal, M.; Sletta, H.; Moreno, S.; Maerk, M.; Christensen, B.E.; Bjerkan, T.; Ellingsen, T.E.; Espin, G.; Ertesvag, H.; Valla, S.
    The Azotobacter vinelandii AlgE mannuronan C-5-epimerase family is essential for the in vivo control of alginate monomer composition and for functional cyst formation (2008), Environ. Microbiol., 10, 1760-1770.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.3.37 extracellular
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Azotobacter vinelandii
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Organism

EC Number Organism UniProt Comment Textmining
5.1.3.37 Azotobacter vinelandii Q44494 isoform AlgE1
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5.1.3.37 Azotobacter vinelandii Q44496 isoform AlgE3
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Synonyms

EC Number Synonyms Comment Organism
5.1.3.37 AlgE3
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Azotobacter vinelandii

General Information

EC Number General Information Comment Organism
5.1.3.37 physiological function generation of a strain in which all the algE genes are inactivated by deletion (algE1-4 and algE1-7) or interruption (algE5). The shake flask-grown mutant strain produces a polymer containing less than 2% G (with periplasmic isoform algG still active), while wild-type alginates contain 25% G. Addition of proteases to growth medium results in a strong increase in the chain lengths of the alginates produced. The mutant strain is unable to form functional cysts. Single algE gene inactivations, with the exception of algE3, has no detected effect on cell growth, morphology or alginate structure Azotobacter vinelandii
5.1.3.37 physiological function generation of a strain in which all the algE genes are inactivated by deletion (algE1-4 and algE6-7) or interruption (algE5). The shake flask-grown mutant strain produces a polymer containing less than 2% G (with periplasmic isoform algG still active), while wild-type alginates contain 25% G. Addition of proteases to growth medium results in a strong increase in the chain lengths of the alginates produced. The mutant strain is unable to form functional cysts. single isoform AlgE3 insertion mutants produce alginates with a G content as low as 8%, and contain almost no GG diads Azotobacter vinelandii