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Literature summary extracted from

  • Thompson, P.R.; Hughes, D.W.; Wright, G.R.
    Mechanism of aminoglycoside 3'-phosphotransferase type IIIa: His188 is not a phosphate-accepting residue (1996), Chem. Biol., 3, 747-755.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.B26 expression in Escherichia coli Enterococcus faecium

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.B26 H123A mutation has minimal effects on phosphorylation of several aminoglycosides Enterococcus faecium
2.7.1.B26 H188A mutation has minimal effects on phosphorylation of several aminoglycosides Enterococcus faecium
2.7.1.B26 H78A mutation has minimal effects on phosphorylation of several aminoglycosides Enterococcus faecium
2.7.1.B26 H82A mutation has minimal effects on phosphorylation of several aminoglycosides Enterococcus faecium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.B26 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide 10 mM, 2% residual activity Enterococcus faecium
2.7.1.B26 4-chloromercuribenzoic acid 0.5 mM, 3% residual activity Enterococcus faecium
2.7.1.B26 diethyldicarbonate 2.5 mM, 10% residual activity Enterococcus faecium
2.7.1.B26 kanamycin A
-
 Enterococcus faecium
2.7.1.B26 N-ethylmaleimide 0.5 mM, 47% residual activity Enterococcus faecium
2.7.1.B26 neomycin B
-
 Enterococcus faecium
2.7.1.B26 Phenylglyoxal 2.5 mM, 10% residual activity Enterococcus faecium
2.7.1.B26 Tetranitromethane 2.5 mM, 23% residual activity Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.B26 0.00029
-
 neomycin B mutant H123A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.00092
-
 kanamycin A mutant H123A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.00116
-
 ATP mutant H123A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.0013
-
 neomycin B mutant H82A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.0024
-
 kanamycin A mutant H82A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.0032
-
 neomycin B mutant H188A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.0051
-
 kanamycin A mutant H78A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.00772
-
 neomycin B wild-type, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.011
-
 ATP mutant H78A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.012
-
 ATP mutant H82A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.0126
-
 kanamycin A wild-type, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.0174
-
 kanamycin A mutant H188A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.027
-
 neomycin B mutant H78A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.0277
-
 ATP wild-type, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.0335
-
 ATP mutant H188A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.093
-
 amikacin mutant H82A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.11
-
 amikacin mutant H123A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.184
-
 amikacin mutant H188A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.206
-
 amikacin mutant H78A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.245
-
 amikacin wild-type, pH 7.5, 37°C Enterococcus faecium

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.B26 Enterococcus faecium E9LMR8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.B26 ATP + amikacin
-
 Enterococcus faecium ADP + amikacin 3'-phosphate
-
 ?
2.7.1.B26 ATP + kanamycin A
-
 Enterococcus faecium ADP + kanamycin A 3'-phosphate
-
 ?
2.7.1.B26 ATP + neomycin B
-
 Enterococcus faecium ADP + neomycin B 3'-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.B26 APH-3'-IIIa
-
 Enterococcus faecium
2.7.1.B26 Aph3
-
 Enterococcus faecium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.B26 0.35
-
 ATP mutant H82A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.35
-
 kanamycin A mutant H82A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.49
-
 kanamycin A mutant H123A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.5
-
 amikacin mutant H82A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.57
-
 ATP mutant H123A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.66
-
 ATP mutant H78A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.67
-
 kanamycin A mutant H78A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.69
-
 neomycin B mutant H82A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.83
-
 neomycin B mutant H123A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 0.87
-
 amikacin mutant H123A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 1.2
-
 neomycin B mutant H188A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 1.74
-
 amikacin mutant H78A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 1.76
-
 ATP wild-type, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 1.79
-
 kanamycin A wild-type, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 2.04
-
 amikacin mutant H188A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 2.08
-
 neomycin B wild-type, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 2.46
-
 amikacin wild-type, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 2.46
-
 neomycin B mutant H78A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 3.36
-
 kanamycin A mutant H188A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 3.75
-
 ATP mutant H188A, pH 7.5, 37°C Enterococcus faecium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.B26 1.68
-
 kanamycin A mutant H78A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 1.84
-
 neomycin B mutant H78A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 2.65
-
 neomycin B wild-type, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 2.74
-
 neomycin B mutant H188A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 5.03
-
 kanamycin A mutant H123A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 6.38
-
 kanamycin A wild-type, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 6.7
-
 kanamycin A mutant H82A, pH 7.5, 37°C Enterococcus faecium
2.7.1.B26 6.95
-
 neomycin B mutant H123A, pH 7.5, 37°C Enterococcus faecium