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Literature summary extracted from

  • Skjak-Braek, G.; Larsen, B.
    Biosynthesis of alginate: Purification and characterisation of mannuronan C-5-epimerase from Azotobacter vinelandii (1985), Carbohydr. Res., 139, 273-283.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.37 0.62
-
 [mannuronan]-beta-D-mannuronate pH 6.8, 30°C, presence of 8.6 mM Ca2+ Azotobacter vinelandii
5.1.3.37 0.9
-
 [mannuronan]-beta-D-mannuronate pH 6.8, 30°C, presence of 0.68 mM Ca2+ Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.37 Ca2+ absolutely required, optimum concentration 1 mM Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.37 120000
-
 1 * 120000, SDS-PAGE Azotobacter vinelandii
5.1.3.37 122000
-
 PAGE Azotobacter vinelandii
5.1.3.37 150000
-
 gel filtration Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.37 Azotobacter vinelandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.37 rapid purification procedure based on fast protein liquid chromatography on MonoQ resin Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.37 [mannuronan]-beta-D-mannuronate
-
 Azotobacter vinelandii [alginate]-alpha-L-guluronate very efficient conversion of poly(mannuronic acid) into a polymer containing 42% of guluronic acid ?

Subunits

EC Number Subunits Comment Organism
5.1.3.37 monomer 1 * 120000, SDS-PAGE Azotobacter vinelandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.37 7
-
-
 Azotobacter vinelandii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.1.3.37 Azotobacter vinelandii isoelectric focusing
-
 4.5