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Literature summary extracted from

  • Saitou, H.; Watanabe, M.; Maruyama, K.
    Molecular and catalytic properties of 2,4-diacetylphloroglucinol hydrolase (PhlG) from Pseudomonas sp. YGJ3 (2012), Biosci. Biotechnol. Biochem., 76, 1239-1241.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.7.1.24 additional information no effect on enzyme activity by 2,2-dipyridine, 8-hydroxyquinoline, 1,1-phenanthroline, tiron, and EDTA Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.24 gene phlG, from locus phlHGFACBDEI, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression in Escherichia coli strain XL-1 Blue Pseudomonas sp.

General Stability

EC Number General Stability Organism
3.7.1.24 2-mercaptoethanol or DTT is required for stability, the purified enzyme is rapidly inactivates without stabilizing compounds, the enzyme can be partially reactivated by 10 mM 2-mercaptoethanol to regain 20% of its initial activity, the enzyme is irreversibly inactivated by freezing and thawing Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.24 Cu2+ slight inhibition Pseudomonas sp.
3.7.1.24 diethyldicarbonate 60% inhibition at 0.5 mM Pseudomonas sp.
3.7.1.24 monoacetylphloroglucinol competitive inhibition Pseudomonas sp.
3.7.1.24 additional information no inhibition by 2-hydroxyacetophenone and 4-hydroxyacetophenone. No effect on enzyme activity by 2,2-dipyridine, 8-hydroxyquinoline, 1,1-phenanthroline, tiron, and EDTA Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.24 0.024
-
 2,4-diacetylphloroglucinol recombinant enzyme, pH 7.0, 25°C Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.7.1.24 Co2+ specifically and significantly activates the enzyme, shows a hyperbolic saturation curve in the micromolar range, binding to the enzyme might induce a conformational change that increases the catalytic effect of Zn2+ or might forma a distinct catalytic site besides that for Zn2+ Pseudomonas sp.
3.7.1.24 additional information the enzyme has a 168HXHXD172 metal binding motif. Poor effects on enzyme activity by Ca2+, Ni2+, Fe3+, Zn2+, and Mg2+ Pseudomonas sp.
3.7.1.24 Zn2+ a zinc hydrolase, located at the bottom of a deep narrow pocket of the enzyme molecule, coordinated by H129, E160, H270, and E274 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.24 35000
-
 2 * 35000, recombinant enzyme, SDS-PAGE Pseudomonas sp.
3.7.1.24 65000
-
 recombinant enzyme, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.24 2,4-diacetylphloroglucinol + H2O Pseudomonas sp.
-
 2-acetylphloroglucinol + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.24 Pseudomonas sp. F7J5X9 gene phlG
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.24 recombinant enzyme 22fold from Escherichia coli strain XL-1 Blue to homogeneity by ammonium sulfate fractionation, gel filtration, two steps of anion exchange chromatography, and hydrophobic interaction chromatograpy Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.24 9.1
-
 purified recombinant enzyme, pH 7.0, 25°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.24 2,4-diacetylphloroglucinol + H2O
-
 Pseudomonas sp. 2-acetylphloroglucinol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.24 homodimer 2 * 35000, recombinant enzyme, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.7.1.24 Phlg
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.7.1.24 25
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.7.1.24 47 55 purified recombinant enzyme, 50% activity remaining at 47°C, inactivation at 55°C Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.24 5.8
-
 2,4-diacetylphloroglucinol recombinant enzyme, pH 7.0, 25°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.24 6
-
-
 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.7.1.24 0.087
-
 monoacetylphloroglucinol recombinant enzyme, pH 7.0, 25°C Pseudomonas sp.

General Information

EC Number General Information Comment Organism
3.7.1.24 physiological function 2,4-diacetylphloroglucinol is produced by Pseudomonas fluorescens an antibiotic that represses the growth of pathogenic microorganisms in the rhizosphere of host plants, the diacetylphloroglucinol hydrolase is a key compound in the biocontrol of Pseudomonas fluorescens Pseudomonas sp.