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Literature summary extracted from
- Characterization of 2,4-dinitrotoluene dioxygenase from recombinant Escherichia coli strain PFJS39: Its direct interaction with vitreoscilla hemoglobin (2010), Bioremediat. J., 14, 38-53.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.24 | 2-mercaptoethanol | required for maximal activity, 292.3% activity at 1% (v/v) | Burkholderia sp. |  |
| 1.14.12.24 | ATP | 124% activity at 0.1 mM | Burkholderia sp. | |
| 1.14.12.24 | FMN | 230.7% activity at 0.1 mM | Burkholderia sp. | |
| 1.14.12.24 | GTP | 116% activity at 0.1 mM | Burkholderia sp. | |
| 1.14.12.24 | lipase | 176% activity at 1 mg per g wet weight of cells | Burkholderia sp. | |
| 1.14.12.24 | NADP+ | 2fold enhancement of activity in the presence of NADP+ | Burkholderia sp. | |
| 1.14.12.24 | phosphatidylcholine | 125% activity at 0.05 mg | Burkholderia sp. | |
| 1.14.12.24 | phosphatidylethanolamine | 144% activity at 0.1 mg per g wet weight of cells | Burkholderia sp. | |
| 1.14.12.24 | Phospholipase A | 125% activity at 0.01 mg | Burkholderia sp. |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.12.24 | expressed in Escherichia coli PFJS39 cells | Burkholderia sp. |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.24 | deoxycholate | about 68% residual activity at 6% (v/v) | Burkholderia sp. | |
| 1.14.12.24 | Dodecyl sulfate | about 40% residual activity at 1% (v/v) | Burkholderia sp. | |
| 1.14.12.24 | FAD | - |
Burkholderia sp. | |
| 1.14.12.24 | spermidine | 80% residual activity at 0.05 mg | Burkholderia sp. | |
| 1.14.12.24 | Triton X-100 | about 50% residual activity at 2% (v/v) | Burkholderia sp. | |
| 1.14.12.24 | Tween 20 | about 36% residual activity at 2% (v/v) | Burkholderia sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.12.24 | 0.05 | - |
O2 | at pH 7.0 and 37°C | Burkholderia sp. | |
| 1.14.12.24 | 0.18 | - |
2,4-dinitrotoluene | at pH 7.0 and 37°C | Burkholderia sp. | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.24 | Fe2+ | required for activity, 238.5% activity at 1 mM | Burkholderia sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.24 | 100000 | - |
gel filtration | Burkholderia sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.24 | 2,4-dinitrotoluene + NADH + H+ + O2 | Burkholderia sp. | - |
4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
| 1.14.12.24 | 2,4-dinitrotoluene + NADH + H+ + O2 | Burkholderia sp. DNT | - |
4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.12.24 | Burkholderia sp. | - |
- |
- |
| 1.14.12.24 | Burkholderia sp. DNT | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.12.24 | ammonium sulfate precipitation, column chromatography, and Sephadex G-100 gel filtration | Burkholderia sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.24 | 2.4 | - |
crude extract, at pH 7.0 and 37°C | Burkholderia sp. |
| 1.14.12.24 | 37.3 | - |
after 15.7fold, at pH 7.0 and 37°C | Burkholderia sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.24 | 2,4-dinitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
| 1.14.12.24 | 2,4-dinitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. DNT | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.12.24 | heterotrimer | 1 * 52000-71000, 1 * 23000-25500, 1 * 12000-16500, SDS-PAGE | Burkholderia sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.12.24 | DNT dioxygenase | - |
Burkholderia sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.24 | 37 | - |
- |
Burkholderia sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.24 | 4 | 45 | activity rapidly diminishes when the enzyme is heated above 45°C. At 20°C, the activity is decreased about 25% compared with 37°C. At 15°C, the activity decreases about 85% compared with 37°C, and at 4-5°C, the activity is totally absent | Burkholderia sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.24 | - |
4 | the enzyme shows 5% to 15% loss in catalytic activity when left at 0-4°C for several weeks | Burkholderia sp. |
| 1.14.12.24 | 25 | 37 | the enzyme is stable for 2 days when incubated with substrate at 37°C, 30°C, and 25°C | Burkholderia sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.24 | 7 | - |
- |
Burkholderia sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.24 | 4 | 10 | the enzyme is stable when incubated at pH 6 and 7, but its optimal activity is at pH 7.0. The activity is decreased significantly when incubated at pH 4.0, 5.0, 8.0, and 10.0 | Burkholderia sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.24 | FAD | - |
Burkholderia sp. | |
| 1.14.12.24 | NAD+ | 2fold enhancement of activity in the presence of NAD+ | Burkholderia sp. | |
| 1.14.12.24 | NADH | 5fold enhancement of activity in the presence of NADH | Burkholderia sp. | |
| 1.14.12.24 | NADP+ | 2fold enhancement of activity in the presence of NADP+ | Burkholderia sp. | |
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