EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.27 | expressed in Escherichia coli DH10B cells | Nostoc sp. |
1.14.19.36 | gene desB, DNA and amino acid sequence determination and analysis, phylogenetic tree, genes desA, desB, and desC are in tandem and have an ORF of 822 bp, 1053 bp, and 1080 bp, respectively, and are separated by intergenic regions, the three genes do not form an operon, RT-PCR enzyme expression analysis | Nostoc sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.19.36 | membrane | transmembrane domains at residues 43-65, 71-93, 200-222, and 231-253 | Nostoc sp. | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.19.27 | 33200 | - |
x * 33200, calculated from amino acid sequence | Nostoc sp. |
1.14.19.36 | 41800 | - |
x * 41800, about, sequence calculation | Nostoc sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.27 | 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Nostoc sp. | - |
1-acyl-2-palmitoleoyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.19.36 | a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Nostoc sp. | - |
a 1-stearidonoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
1.14.19.36 | a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Nostoc sp. | - |
a 1-alpha-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.27 | Nostoc sp. | Q704E9 | - |
- |
1.14.19.36 | Nostoc sp. | Q704F0 | gene desB | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.27 | 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Nostoc sp. | 1-acyl-2-palmitoleoyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.19.36 | a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Nostoc sp. | a 1-stearidonoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
1.14.19.36 | a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Nostoc sp. | a 1-alpha-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.19.27 | ? | x * 33200, calculated from amino acid sequence | Nostoc sp. |
1.14.19.36 | ? | x * 41800, about, sequence calculation | Nostoc sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.27 | DesC2 | - |
Nostoc sp. |
1.14.19.36 | DesB | - |
Nostoc sp. |
1.14.19.36 | Omega 3 acyl-lipid desaturase | - |
Nostoc sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.36 | Ferredoxin | - |
Nostoc sp. |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.19.27 | Nostoc sp. | the transcript of, desC, does not show any up- or down-regulation of the expression of the desaturase gene, up to 150 min after shifting the culture from 25°C to 10°C | additional information |
1.14.19.36 | Nostoc sp. | antarctic psychrotolerant, Nostoc sp. SO-36, when grown at 25°C and then shifted to 10°C, shows an increase in the tri-unsaturated fatty acid [C18:3(9,12,15)] at the expense of mono- [C18:1(9)] and di-unsaturated [C18:2(9,12)] fatty acids, but constitutive expression of desB gene when cultures grown at 25°C are shifted to 10°C | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.19.36 | metabolism | the conversion of the C18 fatty acid to C18:3(9,12,15) is catalyzed in a step wise manner by DesC, DesA, and DesB, respectively | Nostoc sp. |
1.14.19.36 | additional information | antarctic psychrotolerant, Nostoc sp. SO-36, when grown at 25°C and then shifted to 10°C, shows an increase in the tri-unsaturated fatty acid [C18:3(9,12,15)] at the expense of mono- [C18:1(9)] and di-unsaturated [C18:2(9,12)] fatty acids | Nostoc sp. |