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Literature summary extracted from

  • Huang, L.; Liu, Y.; Sun, Y.; Yan, Q.; Jiang, Z.
    Biochemical characterization of a novel L-Asparaginase with low glutaminase activity from Rhizomucor miehei and its application in food safety and leukemia treatment (2014), Appl. Environ. Microbiol., 80, 1561-1569.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.1 food industry reduction of acrylamide level in biscuits and bread Rhizomucor miehei
3.5.1.1 medicine the recombinant enzyme potentiate a lectin's induction of leukemic K562 cell apoptosis Rhizomucor miehei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expressed in Escherichia coli BL21(DE3) cells Rhizomucor miehei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 2-mercaptoethanol 55% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Ag+ 42% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Ba2+ 30% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Co2+ 69% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Cr2+ 37% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Cu2+ 13% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Fe3+ 41% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Hg2+ 24% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 K+ 92% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Mg2+ 28% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Na+ 91% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Ni2+ 85% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 SDS 56% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Sn2+ 87% residual activity at 2 mM Rhizomucor miehei
3.5.1.1 Zn2+ 17% residual activity at 2 mM Rhizomucor miehei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Ca2+ 144% activity at 2 mM Rhizomucor miehei
3.5.1.1 Mn2+ 183% activity at 2 mM Rhizomucor miehei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 72000
-
 2 * 72000, SDS-PAGE Rhizomucor miehei
3.5.1.1 75316
-
 2 * 75316, calculated from amino acid sequence Rhizomucor miehei
3.5.1.1 133700
-
 gel filtration Rhizomucor miehei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Rhizomucor miehei highest specificity for L-asparagine L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O Rhizomucor miehei CAU432 highest specificity for L-asparagine L-aspartate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Rhizomucor miehei W0G253
-
-
3.5.1.1 Rhizomucor miehei CAU432 W0G253
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 Ni-IDA column chromatography Rhizomucor miehei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 0.7797
-
 crude enzyme, at 45°C in 50 mM phosphate buffer (pH 7.0) Rhizomucor miehei
3.5.1.1 1.985
-
 after 2.6fold purification, at 45°C in 50 mM phosphate buffer (pH 7.0) Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 D-asparagine + H2O negligible activity Rhizomucor miehei D-aspartate + NH3
-
 ?
3.5.1.1 D-asparagine + H2O negligible activity Rhizomucor miehei CAU432 D-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O highest specificity for L-asparagine Rhizomucor miehei L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O highest specificity for L-asparagine Rhizomucor miehei CAU432 L-aspartate + NH3
-
 ?
3.5.1.1 L-glutamine + H2O negligible activity Rhizomucor miehei L-glutamate + NH3
-
 ?
3.5.1.1 L-glutamine + H2O negligible activity Rhizomucor miehei CAU432 L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 homodimer 2 * 72000, SDS-PAGE Rhizomucor miehei
3.5.1.1 homodimer 2 * 75316, calculated from amino acid sequence Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 ASNase
-
 Rhizomucor miehei
3.5.1.1 L-asparaginase
-
 Rhizomucor miehei
3.5.1.1 L-asparagine amidohydrolase
-
 Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 45
-
-
 Rhizomucor miehei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 30 65 more than 50% activity between 30 and 65°C Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 45
-
 the recombinant enzyme is stable at 45°C for 30 min Rhizomucor miehei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 676
-
 L-asparagine at pH 7.0 and 45°C Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 7
-
-
 Rhizomucor miehei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 4 8 more than 80% activity between pH 4.0 and 8.0 Rhizomucor miehei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.1 Rhizomucor miehei calculated from amino acid sequence
-
 6.8