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Literature summary extracted from

  • Bottiglieri, M.; Keel, C.
    Characterization of PhlG, a hydrolase that specifically degrades the antifungal compound 2,4-diacetylphloroglucinol in the biocontrol agent Pseudomonas fluorescens CHA0 (2006), Appl. Environ. Microbiol., 72, 418-427.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.7.1.24 agriculture the potent antimicrobial compound 2,4-diacetylphloroglucinol is a major determinant of biocontrol activity of plant-beneficial Pseudomonas fluorescens CHA0 against root diseases caused by fungal pathogens Pseudomonas fluorescens
3.7.1.24 additional information by degrading 2,4-diacetylphloroglucinol to mildly toxic monoacetylphloroglucinol, PhlG may help avoid accumulation of a metabolite that at high levels may become toxic to the producing bacterium, as has been observed for strains CHA0 and F11 Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.24 gene phlG, located upstream of the phlACBD biosynthetic operon, between the phlF and phlH genes which encode pathway-specific regulators, genetic structure of the operon, functional recombinant expression of soluble His6-tagged enzyme in Escherichia coli strain BL21(DE3)/pME8032 Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
3.7.1.24 additional information construction of the DELTAphlG mutant CHA1091 and DELTAphlA/DELTAphlG double mutant Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.24 additional information no inhibition by monoacetylphloroglucinol, triacetylphloroglucinol, or salicylate Pseudomonas fluorescens
3.7.1.24 pyoluteorin strong inhibition of the enzyme, another antifungal compound produced by the bacterium Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.24 0.14
-
 2,4-diacetylphloroglucinol recombinant enzyme, pH 7.0, 30°C Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.24 40000
-
 x * 40000, recombinant His-tagged enzyme, SDS-PAGE Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.24 2,4-diacetylphloroglucinol + H2O Pseudomonas fluorescens the enzyme functions as a hydrolase specifically degrades 2,4-diacetylphloroglucinol, DAPG, to equimolar amounts of mildly toxic monoacetylphloroglucinol (MAPG) and acetate 2-acetylphloroglucinol + acetate
-
 ?
3.7.1.24 2,4-diacetylphloroglucinol + H2O Pseudomonas fluorescens CHA0 the enzyme functions as a hydrolase specifically degrades 2,4-diacetylphloroglucinol, DAPG, to equimolar amounts of mildly toxic monoacetylphloroglucinol (MAPG) and acetate 2-acetylphloroglucinol + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.24 Pseudomonas fluorescens A0A2C9EVE6 gene phlG
-
3.7.1.24 Pseudomonas fluorescens CHA0 A0A2C9EVE6 gene phlG
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.24 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)/pME8032 by nickel affinity chromatography Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.24 2,4-diacetylphloroglucinol + H2O
-
 Pseudomonas fluorescens 2-acetylphloroglucinol + acetate
-
 ?
3.7.1.24 2,4-diacetylphloroglucinol + H2O the enzyme functions as a hydrolase specifically degrades 2,4-diacetylphloroglucinol, DAPG, to equimolar amounts of mildly toxic monoacetylphloroglucinol (MAPG) and acetate Pseudomonas fluorescens 2-acetylphloroglucinol + acetate
-
 ?
3.7.1.24 2,4-diacetylphloroglucinol + H2O
-
 Pseudomonas fluorescens CHA0 2-acetylphloroglucinol + acetate
-
 ?
3.7.1.24 2,4-diacetylphloroglucinol + H2O the enzyme functions as a hydrolase specifically degrades 2,4-diacetylphloroglucinol, DAPG, to equimolar amounts of mildly toxic monoacetylphloroglucinol (MAPG) and acetate Pseudomonas fluorescens CHA0 2-acetylphloroglucinol + acetate
-
 ?
3.7.1.24 additional information the PhlG enzyme does not degrade other compounds with structures similar to diacetylphloroglucinol, such as monoacetylphloroglucinol and triacetylphloroglucinol, or salicylate, and 2,6-dihydroxyacetophenone, suggesting strict substrate specificity Pseudomonas fluorescens ?
-
 ?
3.7.1.24 additional information the PhlG enzyme does not degrade other compounds with structures similar to diacetylphloroglucinol, such as monoacetylphloroglucinol and triacetylphloroglucinol, or salicylate, and 2,6-dihydroxyacetophenone, suggesting strict substrate specificity Pseudomonas fluorescens CHA0 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.24 ? x * 40000, recombinant His-tagged enzyme, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
3.7.1.24 DAPG hydrolase
-
 Pseudomonas fluorescens
3.7.1.24 Phlg
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.7.1.24 30
-
 assay at Pseudomonas fluorescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.7.1.24 16 42 50% of maximal activity within this range, optimum at 30°C Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.24 33
-
 2,4-diacetylphloroglucinol recombinant enzyme, pH 7.0, 30°C Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.24 7
-
 assay at Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.7.1.24 6 8 10% of maximal activity at pH 7.6, inactive at pH 8.0 Pseudomonas fluorescens

Expression

EC Number Organism Comment Expression
3.7.1.24 Pseudomonas fluorescens expression of gene phlG is not influenced by the substrate 2,4-diacetylphloroglucinol or the degradation product monoacetylphloroglucinol but is subject to positive control by the GacS/GacA two-component system and to negative control by the pathway-specific regulators PhlF and PhlH down
3.7.1.24 Pseudomonas fluorescens gene phlG expression regulation study, overview additional information

General Information

EC Number General Information Comment Organism
3.7.1.24 evolution both phloretin hydrolase, EC 3.7.1.4, and 2,4-diacetylphloroglucinol hydrolase show no sequence similarity to other known C-C hydrolase enzymes and contain no motifs typical of the alpha/beta-hydrolase-fold superfamily Pseudomonas fluorescens
3.7.1.24 malfunction 2,4-diacetylphloroglucinol added to cultures of a DAPG-negative DELTAphlA mutant of strain CHA0 is completely degraded, and MAPG is temporarily accumulated. In contrast, 2,4-diacetylphloroglucinol is not degraded in cultures of a DELTAphlA/DELTAphlG double mutant Pseudomonas fluorescens
3.7.1.24 metabolism the potent antimicrobial compound 2,4-diacetylphloroglucinol is a major determinant of biocontrol activity of plant-beneficial Pseudomonas fluorescens CHA0 against root diseases caused by fungal pathogens Pseudomonas fluorescens
3.7.1.24 physiological function the hydrolase is required for specific degradation of the antifungal compound 2,4-diacetylphloroglucinol in the biocontrol agent Pseudomonas fluorescens CHA0, it catalyzes the conversion of the potent antibiotic DAPG to less-toxic MAPG, with a high specificity for its substrate Pseudomonas fluorescens