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Literature summary extracted from

  • Parales, R.; Huang, R.; Yu, C.; Parales, J.; Lee, F.; Lessner, D.; Ivkovic-Jensen, M.; Liu, W.; Friemann, R.; Ramaswamy, S.; Gibson, D.
    Purification, characterization, and crystallization of the components of the nitrobenzene and 2-nitrotoluene dioxygenase enzyme systems (2005), Appl. Environ. Microbiol., 71, 3806-3814.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.12.23 oxygenase component, to 1.2 A resolution, hexagonal space group P63 with cell dimensions a = b = 121.6 A and c = 84.4 A Acidovorax sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.23 0.02
-
 2-nitrotoluene pH 6.8, 30°C Acidovorax sp.
1.14.12.23 0.121
-
 Naphthalene pH 6.8, 30°C Acidovorax sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.23 25300
-
 3 * 51200, alpha-subunit, 3 * 25300, beta-subunit, SDS-PAGE of oxygenase component Acidovorax sp.
1.14.12.23 25500
-
 3 * 50400, alpha-subunit, 3 * 25500, beta-subunit, SDS-PAGE of oxygenase component Acidovorax sp.
1.14.12.23 50400
-
 3 * 50400, alpha-subunit, 3 * 25500, beta-subunit, SDS-PAGE of oxygenase component Acidovorax sp.
1.14.12.23 51200
-
 3 * 51200, alpha-subunit, 3 * 25300, beta-subunit, SDS-PAGE of oxygenase component Acidovorax sp.
1.14.12.23 210000
-
 gel filtration, oxygenase component Acidovorax sp.
1.14.12.23 215900
-
 dynamic light scattering, oxygenase component Acidovorax sp.
1.14.12.23 216000
-
 gel filtration, oxygenase component Acidovorax sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.23 Acidovorax sp.
-
-
-
1.14.12.23 Acidovorax sp. JS42
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.23 2-nitrotoluene + NADH + O2
-
 Acidovorax sp. 3-methylcatechol + nitrite + NAD+
-
 ?
1.14.12.23 2-nitrotoluene + NADH + O2
-
 Acidovorax sp. JS42 3-methylcatechol + nitrite + NAD+
-
 ?
1.14.12.23 naphthalene + NADH + O2
-
 Acidovorax sp. ? + nitrite + NAD+
-
 ?
1.14.12.23 naphthalene + NADH + O2
-
 Acidovorax sp. JS42 ? + nitrite + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.23 hexamer 3 * 50400, alpha-subunit, 3 * 25500, beta-subunit, SDS-PAGE of oxygenase component Acidovorax sp.
1.14.12.23 hexamer 3 * 51200, alpha-subunit, 3 * 25300, beta-subunit, SDS-PAGE of oxygenase component Acidovorax sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.12.23 2.3
-
 2-nitrotoluene pH 6.8, 30°C Acidovorax sp.
1.14.12.23 2.4
-
 Naphthalene pH 6.8, 30°C Acidovorax sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.23 NADH 0.5 mol of NADH is required to reduce each alphabeta heterodimer of oxygenase Acidovorax sp.
1.14.12.23 [2Fe-2S]-center presence of a Rieske [2Fe-2S] cluster in the oxygenase component Acidovorax sp.