EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.12.24 | expressed in Escherichia coli TG1 cells | Burkholderia cepacia |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.12.24 | V350F | mutant displays significantly increased activity towards o-nitrophenol (47 times), m-nitrophenol (34 times), and o-methoxyphenol (174 times) as well as an expanded substrate range that now includes m-methoxyphenol, o-cresol, and m-cresol | Burkholderia sp. |
1.14.12.24 | V350F | the mutant of the alpha subunit is inactive with 2,4-dinitrotoluene but shows significantly increased activity towards 2-nitrophenol (47times), 3-nitrophenol (34times), and 2-methoxyphenol (174times) as well as an expanded substrate range that now includes 3-methoxyphenol, o-cresol, and m-cresol (wild type enzyme has no detectable activity for these substrates). The mutant also exhibits 10fold enhanced activity towards naphthalene forming (1R,2S)-cis-1,2-dihydro-1,2-dihydroxynaphthalene | Burkholderia cepacia |
1.14.12.24 | V350M | mutant displays increased activity towards o-nitrophenol (20 times) and o-methoxyphenol (162 times) as well as novel activity towards o-cresol | Burkholderia sp. |
1.14.12.24 | V350M | the mutant is inactive with 2,4-dinitrotoluene but displays increased activity towards 2-nitrophenol (20times) and 2-methoxyphenol (162times) as well as novel activity towards 2-cresol compared to the wild type enzyme. The mutant also exhibits 10fold enhanced activity towards naphthalene forming (1R,2S)-cis-1,2-dihydro-1,2-dihydroxynaphthalene | Burkholderia cepacia |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.24 | 2,4-dinitrotoluene + NADH + O2 | Burkholderia cepacia | highest activity | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
1.14.12.24 | 2,4-dinitrotoluene + NADH + O2 | Burkholderia cepacia R34 | highest activity | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.24 | Burkholderia cepacia | - |
- |
- |
1.14.12.24 | Burkholderia cepacia R34 | - |
- |
- |
1.14.12.24 | Burkholderia sp. | Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 | Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd | - |
1.14.12.24 | Burkholderia sp. DNT | Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 | Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.24 | 2,4-dinitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
1.14.12.24 | 2,4-dinitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. DNT | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
1.14.12.24 | 2,4-dinitrotoluene + NADH + O2 | highest activity | Burkholderia cepacia | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
1.14.12.24 | 2,4-dinitrotoluene + NADH + O2 | highest activity | Burkholderia cepacia R34 | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
1.14.12.24 | 2-methoxyphenol + NADH + O2 | - |
Burkholderia cepacia | methoxyhydroquinone + NAD+ + H2O | - |
? | |
1.14.12.24 | 2-methoxyphenol + NADH + O2 | - |
Burkholderia cepacia R34 | methoxyhydroquinone + NAD+ + H2O | - |
? | |
1.14.12.24 | 2-nitrophenol + NADH + O2 | - |
Burkholderia cepacia | nitrohydroquinone + 3-nitrocatechol + NAD+ + H2O | - |
? | |
1.14.12.24 | 2-nitrophenol + NADH + O2 | - |
Burkholderia cepacia R34 | nitrohydroquinone + 3-nitrocatechol + NAD+ + H2O | - |
? | |
1.14.12.24 | 3-nitrophenol + NADH + O2 | - |
Burkholderia cepacia | 4-nitrocatechol + NAD+ + H2O | - |
? | |
1.14.12.24 | 3-nitrophenol + NADH + O2 | - |
Burkholderia cepacia R34 | 4-nitrocatechol + NAD+ + H2O | - |
? | |
1.14.12.24 | m-cresol + NADH + H+ + O2 | - |
Burkholderia sp. | methylhydroquinone + nitrite + NAD+ | no substrate for wild-type. Mutant V350F produces methylhydroquinone at 0.43 nmol/min/mg of protein | ? | |
1.14.12.24 | m-cresol + NADH + H+ + O2 | - |
Burkholderia sp. DNT | methylhydroquinone + nitrite + NAD+ | no substrate for wild-type. Mutant V350F produces methylhydroquinone at 0.43 nmol/min/mg of protein | ? | |
1.14.12.24 | m-methoxyphenol + NADH + H+ + O2 | - |
Burkholderia sp. | methoxyhydroquinone + nitrite + NAD+ | no substrate for wild-type. Mutant V350F produces methoxyhydroquinone at 0.55 nmol/min/mg of protein | ? | |
1.14.12.24 | m-methoxyphenol + NADH + H+ + O2 | - |
Burkholderia sp. DNT | methoxyhydroquinone + nitrite + NAD+ | no substrate for wild-type. Mutant V350F produces methoxyhydroquinone at 0.55 nmol/min/mg of protein | ? | |
1.14.12.24 | m-nitrophenol + NADH + H+ + O2 | - |
Burkholderia sp. | 4-nitrocatechol + nitrite + NAD+ | no substrate for wild-type. Mutant V350F produces 4-nitrocatechol at 0.29 nmol/min/mg of protein | ? | |
1.14.12.24 | m-nitrophenol + NADH + H+ + O2 | - |
Burkholderia sp. DNT | 4-nitrocatechol + nitrite + NAD+ | no substrate for wild-type. Mutant V350F produces 4-nitrocatechol at 0.29 nmol/min/mg of protein | ? | |
1.14.12.24 | additional information | no activity with 2-cresol, 3-methoxyphenol | Burkholderia cepacia | ? | - |
? | |
1.14.12.24 | additional information | no activity with 2-cresol, 3-methoxyphenol | Burkholderia cepacia R34 | ? | - |
? | |
1.14.12.24 | naphthalene + NADH + H+ + O2 | - |
Burkholderia sp. | (1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+ | mutants V350F and V350M exhibit 10fold enhanced activity towards naphthalene | ? | |
1.14.12.24 | naphthalene + NADH + H+ + O2 | - |
Burkholderia sp. DNT | (1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+ | mutants V350F and V350M exhibit 10fold enhanced activity towards naphthalene | ? | |
1.14.12.24 | naphthalene + NADH + O2 | - |
Burkholderia cepacia | (1R,3S)-cis-1,2-dihydro-1,2-dihydroxynaphthalene + NAD+ + H2O | - |
? | |
1.14.12.24 | o-cresol + NADH + H+ + O2 | - |
Burkholderia sp. | methylhydroquinone + 2-hydroxybenzyl alcohol + nitrite + NAD+ | mutant V350F produces methylhydroquinone at 1.52 nmol/min/mg of protein and 2-hydroxybenzyl alcohol at 0.74 nmol/min/mg of protein. Mutant V350M produces methylhydroquinone at 1.97 nmol/min/mg of protein and 2-hydroxybenzyl alcohol at 0.11 nmol/min/mg of protein | ? | |
1.14.12.24 | o-methoxyphenol + NADH + H+ + O2 | - |
Burkholderia sp. | methoxyhydroquinone + nitrite + NAD+ | mutant V350F produces methoxyhydroquinone at 2.5 nmol/min/mg of protein. Mutant V350M produces methoxyhydroquinone at 2.4 mol/min/mg of protein | ? | |
1.14.12.24 | o-nitrophenol + NADH + H+ + O2 | - |
Burkholderia sp. | nitrohydroquinone + 3-nitrocatechol + nitrite + NAD+ | product of wild-type. Mutant V350F produces both nitrohydroquinone at a rate of 0.75 nmol/min/mg of protein and 3-nitrocatechol at a rate of 0.069 nmol/min/mg of protein from o-nitrophenol. Mutant V350M produces both nitrohydroquinone at 0.33 nmol/min/mg of protein and 3-nitrocatechol at 0.089 nmol/min/mg of protein | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.12.24 | DDO | - |
Burkholderia cepacia |
1.14.12.24 | DntAc | alpha subunit | Burkholderia cepacia |