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Literature summary extracted from

  • Boersma, Y.L.; Newman, J.; Adams, T.E.; Cowieson, N.; Krippner, G.; Bozaoglu, K.; Peat, T.S.
    The structure of vanin 1: a key enzyme linking metabolic disease and inflammation (2014), Acta Crystallogr. Sect. D, 70, 3320-3329.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.92 transient expression of soluble FLAG-tagged truncated enzyme mutant, and mutant enzymes E249Q and E439Q in HEK-293T cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.92 purified recombinant FLAG-tagged truncated enzyme, and enzyme mutants E249Q and E439Q, free and in complex with pantothenic acid or inhibitor RR6, sitting drop vapor diffusion method, mixing of 200 nl of 12 mg/ml kifunensine-treated vanin 1 protein in 50 mM bis-tris pH 6.5, 50 mM NaCl, with 200 nl of reservoir solution containing 21% w/v PEG 1500, 10% v/v malate-MES-Tris, pH 6.3, with or without 1 mM pantothenic acid or inhibitor RR6, 25°C, 40-50 days, or 4 weeks after microseeding, X-ray diffraction structure determination and analysis at 2.25 A resolution, molecular replacement and modeling Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.92 E249Q site-directed mutagenesis Homo sapiens
3.5.1.92 E439Q site-directed mutagenesis Homo sapiens
3.5.1.92 E79A site-directed mutagenesis Homo sapiens
3.5.1.92 K178A site-directed mutagenesis Homo sapiens
3.5.1.92 additional information construction of a truncated mutant comprising amino acids 22-493, deleting the native signal sequence and the C-terminal GPI-anchor sequence, and cloning downstream of the mouse interleukin-3 signal peptide in the mammalian expression vector pAPEX-3P and a FLAG tag DYDDDDK Homo sapiens
3.5.1.92 T26I naturally occuring variant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.92 (R)-2,4-dihydroxy-3,3-dimethyl-N-(3-oxo-4-phenylbutyl)butanamide i.e. RR6 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.92 cell surface a glycosylphosphatidylinositol (GPI)-anchored ectoenzyme Homo sapiens 9986
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.92 (R)-pantetheine + H2O Homo sapiens
-
 (R)-pantothenate + 2-aminoethanethiol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.92 Homo sapiens O95497
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.92 glycolipoprotein a glycosylphosphatidylinositol (GPI)-anchored ectoenzyme Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.92 recombinant soluble FLAG-tagged truncated enzyme mutant from 293 cells by immunoaffinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.92 (R)-pantetheine + H2O
-
 Homo sapiens (R)-pantothenate + 2-aminoethanethiol
-
 ?
3.5.1.92 N-(4-methyl-2-oxo-2H-chromen-7-yl)-D-pantothenamide + H2O
-
 Homo sapiens (R)-pantothenate + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.92 oligomer the residues important for oligomerization are Gln179-Phe182, Pro191-Glu193, Phe212-Asp218, Pro243-His251 and Leu326-Asn330 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.5.1.92 pantetheinase
-
 Homo sapiens
3.5.1.92 vanin 1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.92 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.92 8
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.5.1.92 metabolism key enzyme linking metabolic disease and inflammation Homo sapiens