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Literature summary extracted from

  • Ye, S.; Xia, H.; Dong, C.; Cheng, Z.; Xia, X.; Zhang, J.; Zhou, X.; Hu, Y.
    Identification and characterization of Iflavirus 3C-like protease processing activities (2012), Virology, 428, 136-145.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.28 expressed in Escherichia coli BL21(DE3) cells Ectropis obliqua virus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.28 C238A inactive Ectropis obliqua virus
3.4.22.28 D2299A the mutant shows much weaker autocatalytic processing activity compared to the wild type Ectropis obliqua virus
3.4.22.28 H2261A inactive Ectropis obliqua virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.28 4-chloromercuribenzenesulfonate strong inhibition at 0.05 mM Ectropis obliqua virus
3.4.22.28 Cu2+ 5 mM decreases protease activity remarkably relative to the level of activity before the extra cations were added Ectropis obliqua virus
3.4.22.28 Hg2+ 5 mM decreases protease activity remarkably relative to the level of activity before the extra cations were added Ectropis obliqua virus
3.4.22.28 methyl methanethiosulfonate almost complete inhibition at 0.05 mM Ectropis obliqua virus
3.4.22.28 N-ethylmaleimide almost complete inhibition at 0.05 mM Ectropis obliqua virus
3.4.22.28 Na+ the enzyme is strongly inhibited by 200 mM Na+ Ectropis obliqua virus
3.4.22.28 phenylmethylsulfonyl fluoride
-
 Ectropis obliqua virus
3.4.22.28 Zn2+ 5 mM decreases protease activity remarkably relative to the level of activity before the extra cations were added Ectropis obliqua virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.28 additional information Mn2+ or Ca2+ do not affect protease activity Ectropis obliqua virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.28 34000
-
 x * 34000, SDS-PAGE Ectropis obliqua virus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.28 Ectropis obliqua virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.28 Ni-NTA column chromatography Ectropis obliqua virus

Subunits

EC Number Subunits Comment Organism
3.4.22.28 ? x * 34000, SDS-PAGE Ectropis obliqua virus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.28 3C-like protease
-
 Ectropis obliqua virus
3.4.22.28 3CL
-
 Ectropis obliqua virus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.28 22
-
-
 Ectropis obliqua virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.28 7.5
-
-
 Ectropis obliqua virus