EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.22.70 | additional information | sortase A mediates site-specific immobilization for identification of protein interactions in affinity purification-mass spectrometry experiments, overview. the enzyme is used for proteins or peptide baits with a C-terminal LPXTG recognition motif that are covalently immobilized to beads carrying an oligoglycine peptide (nucleophile). Tryptic on-bead digestion and spike-in of a heavy isotope labeled reference peptide (13C515N1-Val) allows for the absolute quantification of immobilized peptide or protein by MALDI-MS, method overview | Staphylococcus aureus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.70 | Staphylococcus aureus | - |
- |
- |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.22.70 | additional information | sortase A mediates site-specific immobilization for identification of protein interactions in affinity purification-mass spectrometry experiments, overview | Staphylococcus aureus |