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Literature summary extracted from

  • Takasuka, T.E.; Bianchetti, C.M.; Tobimatsu, Y.; Bergeman, L.F.; Ralph, J.; Fox, B.G.
    Structure-guided analysis of catalytic specificity of the abundantly secreted chitosanase SACTE_5457 from Streptomyces sp. SirexAA-E (2014), Proteins, 82, 1245-1257.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 expressed in Escherichia coli BL21(DE3) cells Streptomyces sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.132 sitting drop vapor diffusion method, using 10% (w/v) MEPEG 5K, 300 mM tetramethylammonium chloride, and 100 mM Bis-Tris, pH 6.5, at 4°C Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Streptomyces sp.
-
 ?
-
 ?
3.2.1.132 chitosan + H2O Streptomyces sp. SirexAA-E
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Streptomyces sp. G2NRC4
-
-
3.2.1.132 Streptomyces sp. SirexAA-E G2NRC4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 HisTrap column chromatography, and gel filtration Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Streptomyces sp. ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Streptomyces sp. SirexAA-E ?
-
 ?
3.2.1.132 additional information the enzyme does not react with chitin. Oligosaccharides with degree of polymerization above 4 are not hydrolyzed Streptomyces sp. ?
-
 ?
3.2.1.132 additional information the enzyme does not react with chitin. Oligosaccharides with degree of polymerization above 4 are not hydrolyzed Streptomyces sp. SirexAA-E ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 SACTE_5457
-
 Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.132 6.5
-
 chitosan chitosan with 75-85% deacetylation, at pH 6.0 and 45°C Streptomyces sp.
3.2.1.132 10
-
 chitosan chitosan with less than 75% deacetylation, at pH 6.0 and 45°C Streptomyces sp.
3.2.1.132 11.2
-
 chitosan chitosan with more than 90% deacetylation, at pH 6.0 and 45°C Streptomyces sp.