EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.21.25 | food industry | the high milk-clotting ability of religiosin C supports its use in the food industry | Ficus religiosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.25 | acetonitrile | 60.03% residual activity at 50% (v/v) | Ficus religiosa | |
3.4.21.25 | butanol | 68.24% residual activity at 50% (v/v) | Ficus religiosa | |
3.4.21.25 | chymostatin | 2.45% residual activity at 5 mM | Ficus religiosa | |
3.4.21.25 | diisopropylfluorophosphate | 2.34% residual activity at 1 mM | Ficus religiosa | |
3.4.21.25 | dioxane | 65.35% residual activity at 50% (v/v) | Ficus religiosa | |
3.4.21.25 | ethanol | 75.09% residual activity at 50% (v/v) | Ficus religiosa | |
3.4.21.25 | additional information | not inhibited by EDTA, EGTA, iodoacetic acid, 2-phenanthroline, SBTI, and dithiothreitol | Ficus religiosa | |
3.4.21.25 | phenylmethylsulfonyl fluoride | 5.12% residual activity at 5 mM | Ficus religiosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.25 | 0.002 | - |
L-alanyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa | |
3.4.21.25 | 0.004 | - |
L-alanyl-L-alanyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa | |
3.4.21.25 | 0.093 | - |
L-leucyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa | |
3.4.21.25 | 0.273 | - |
L-gamma-glutamyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.25 | additional information | the enzyme is not influenced by Hg2+, Na+, K+, Mg2+, Ca2+, Rb+, and Li+ | Ficus religiosa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.25 | 80000 | - |
1 * 80000, SDS-PAGE | Ficus religiosa |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.4.21.25 | additional information | the enzyme is not influenced by urea, methanol, and guanidine hydrochloride | Ficus religiosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.25 | Ficus religiosa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.25 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration | Ficus religiosa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.21.25 | latex | - |
Ficus religiosa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.21.25 | additional information | - |
enzyme from crude latex shows a specific activity of 23.99 units/mg at pH 8.0 and 37°C. After 2.29fold purification, the enzyme shows a specific activity of 55.01 units/mg at pH 8.0 and 37°C. One unit of enzyme activity is defined as the amount of enzyme that gives rise to an increase in one unit of absorbance at 280 nm per min of casein digestion | Ficus religiosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.25 | casein + H2O | - |
Ficus religiosa | ? | - |
? | |
3.4.21.25 | L-alanyl-4-nitroanilide + H2O | - |
Ficus religiosa | L-alanine + 4-nitroaniline | - |
? | |
3.4.21.25 | L-alanyl-L-alanyl-4-nitroanilide + H2O | - |
Ficus religiosa | L-alanyl-L-alanine + 4-nitroaniline | - |
? | |
3.4.21.25 | L-gamma-glutamyl-4-nitroanilide + H2O | - |
Ficus religiosa | L-glutamate + 4-nitroaniline | - |
? | |
3.4.21.25 | L-leucyl-4-nitroanilide + H2O | - |
Ficus religiosa | L-leucine + 4-nitroaniline | - |
? | |
3.4.21.25 | additional information | no activity toward Nalpha-benzoyl-DL-arginyl-4-nitroanilide and N-succinyl-L-phenylalanyl-4-nitroanilide | Ficus religiosa | ? | - |
? | |
3.4.21.25 | skim milk + H2O | - |
Ficus religiosa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.21.25 | monomer | 1 * 80000, SDS-PAGE | Ficus religiosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.25 | religiosin C | - |
Ficus religiosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.25 | 45 | 50 | - |
Ficus religiosa |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.25 | 40 | 55 | more than 80% of the activity is observed from 40 to 55°C. The activity decreases steadily as the temperature rises over 60°C and no activity is observed at 80°C | Ficus religiosa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.25 | 20 | 70 | the enzyme shows more than 90% activity up to 60°C for 30 min. 87% of activity is observed at 70°C when incubated for 30 min | Ficus religiosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.25 | 25.48 | - |
L-alanyl-L-alanyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa | |
3.4.21.25 | 32.13 | - |
L-alanyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa | |
3.4.21.25 | 207.8 | - |
L-gamma-glutamyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa | |
3.4.21.25 | 749.7 | - |
L-leucyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.25 | 6 | 8 | - |
Ficus religiosa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.25 | 3.5 | 11.5 | the enzyme retains more than 80% of activity from pH 3.5 to 11.5. No activity is observed at or below pH 2.0 | Ficus religiosa |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.25 | 4 | 11.5 | the enzyme shows more than 90% activity when incubated at pH 4.5 to 11.5 for 30 min | Ficus religiosa |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.4.21.25 | Ficus religiosa | isoelectric focusing | - |
4.6 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.25 | 422.2 | - |
L-leucyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa | |
3.4.21.25 | 749.7 | - |
L-gamma-glutamyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa | |
3.4.21.25 | 6888 | - |
L-alanyl-L-alanyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa | |
3.4.21.25 | 13910 | - |
L-alanyl-4-nitroanilide | at pH 8.0 and 37°C | Ficus religiosa |