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Literature summary extracted from

  • Sharma, A.; Kumari, M.; Jagannadham, M.
    Religiosin C, a cucumisin-like serine protease from Ficus religiosa (2012), Process Biochem., 47, 914-921.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.21.25 food industry the high milk-clotting ability of religiosin C supports its use in the food industry Ficus religiosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.25 acetonitrile 60.03% residual activity at 50% (v/v) Ficus religiosa
3.4.21.25 butanol 68.24% residual activity at 50% (v/v) Ficus religiosa
3.4.21.25 chymostatin 2.45% residual activity at 5 mM Ficus religiosa
3.4.21.25 diisopropylfluorophosphate 2.34% residual activity at 1 mM Ficus religiosa
3.4.21.25 dioxane 65.35% residual activity at 50% (v/v) Ficus religiosa
3.4.21.25 ethanol 75.09% residual activity at 50% (v/v) Ficus religiosa
3.4.21.25 additional information not inhibited by EDTA, EGTA, iodoacetic acid, 2-phenanthroline, SBTI, and dithiothreitol Ficus religiosa
3.4.21.25 phenylmethylsulfonyl fluoride 5.12% residual activity at 5 mM Ficus religiosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.25 0.002
-
L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
3.4.21.25 0.004
-
L-alanyl-L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
3.4.21.25 0.093
-
L-leucyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
3.4.21.25 0.273
-
L-gamma-glutamyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.25 additional information the enzyme is not influenced by Hg2+, Na+, K+, Mg2+, Ca2+, Rb+, and Li+ Ficus religiosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.25 80000
-
1 * 80000, SDS-PAGE Ficus religiosa

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.21.25 additional information the enzyme is not influenced by urea, methanol, and guanidine hydrochloride Ficus religiosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.25 Ficus religiosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.25 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration Ficus religiosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.25 latex
-
Ficus religiosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.25 additional information
-
enzyme from crude latex shows a specific activity of 23.99 units/mg at pH 8.0 and 37°C. After 2.29fold purification, the enzyme shows a specific activity of 55.01 units/mg at pH 8.0 and 37°C. One unit of enzyme activity is defined as the amount of enzyme that gives rise to an increase in one unit of absorbance at 280 nm per min of casein digestion Ficus religiosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.25 casein + H2O
-
Ficus religiosa ?
-
?
3.4.21.25 L-alanyl-4-nitroanilide + H2O
-
Ficus religiosa L-alanine + 4-nitroaniline
-
?
3.4.21.25 L-alanyl-L-alanyl-4-nitroanilide + H2O
-
Ficus religiosa L-alanyl-L-alanine + 4-nitroaniline
-
?
3.4.21.25 L-gamma-glutamyl-4-nitroanilide + H2O
-
Ficus religiosa L-glutamate + 4-nitroaniline
-
?
3.4.21.25 L-leucyl-4-nitroanilide + H2O
-
Ficus religiosa L-leucine + 4-nitroaniline
-
?
3.4.21.25 additional information no activity toward Nalpha-benzoyl-DL-arginyl-4-nitroanilide and N-succinyl-L-phenylalanyl-4-nitroanilide Ficus religiosa ?
-
?
3.4.21.25 skim milk + H2O
-
Ficus religiosa ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.21.25 monomer 1 * 80000, SDS-PAGE Ficus religiosa

Synonyms

EC Number Synonyms Comment Organism
3.4.21.25 religiosin C
-
Ficus religiosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.25 45 50
-
Ficus religiosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.25 40 55 more than 80% of the activity is observed from 40 to 55°C. The activity decreases steadily as the temperature rises over 60°C and no activity is observed at 80°C Ficus religiosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.25 20 70 the enzyme shows more than 90% activity up to 60°C for 30 min. 87% of activity is observed at 70°C when incubated for 30 min Ficus religiosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.25 25.48
-
L-alanyl-L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
3.4.21.25 32.13
-
L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
3.4.21.25 207.8
-
L-gamma-glutamyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
3.4.21.25 749.7
-
L-leucyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.25 6 8
-
Ficus religiosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.25 3.5 11.5 the enzyme retains more than 80% of activity from pH 3.5 to 11.5. No activity is observed at or below pH 2.0 Ficus religiosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.25 4 11.5 the enzyme shows more than 90% activity when incubated at pH 4.5 to 11.5 for 30 min Ficus religiosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.25 Ficus religiosa isoelectric focusing
-
4.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.25 422.2
-
L-leucyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
3.4.21.25 749.7
-
L-gamma-glutamyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
3.4.21.25 6888
-
L-alanyl-L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa
3.4.21.25 13910
-
L-alanyl-4-nitroanilide at pH 8.0 and 37°C Ficus religiosa