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Literature summary extracted from

  • Sakamoto, T.; Los, D.A.; Higashi, S.; Wada, H.; Nishida, I.; Ohmori, M.; Murata, N.
    Cloning of omega 3 desaturase from cyanobacteria and its use in altering the degree of membrane-lipid unsaturation (1994), Plant Mol. Biol., 26, 249-263.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.36 the desA gene, which encodes the DELTA12 desaturase of Synechocystis sp. PCC 6803, and the desB gene are introduced into Synechococcus sp. PCC 7942. Whilst the parent cyanobacterium can only desaturate membrane lipids at the DELTA9 position of fatty acids, the resultant transformant is able to desaturate fatty acids of membrane lipids at the DELTA9, DELTA12 and omega3 positions Synechocystis sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.36 additional information the desB gene in Synechocystis sp. PCC 6803 is mutated by insertion of a kanamycin-resistance gene cartridge. The resultant mutant is unable to desaturate fatty acids at the omega3 position Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.36 41900
-
 x * 41900, SDS-PAGE Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.36 a 1-gamma-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ Synechocystis sp.
-
 a 1-stearidonoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O
-
 ?
1.14.19.36 a 1-linoleoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ Synechocystis sp.
-
 a 1-alpha-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.36 Synechocystis sp. Q55240
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.19.36 a 1-gamma-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ = a 1-stearidonoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O (1) Synechocystis sp.
a
1.14.19.36 a 1-linoleoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ = a 1-alpha-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O (2) Synechocystis sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.36 a 1-gamma-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+
-
 Synechocystis sp. a 1-stearidonoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O
-
 ?
1.14.19.36 a 1-linoleoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+
-
 Synechocystis sp. a 1-alpha-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.36 ? x * 41900, SDS-PAGE Synechocystis sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.19.36 DesB
-
 Synechocystis sp.

Expression

EC Number Organism Comment Expression
1.14.19.36 Synechocystis sp. the level of the desB transcript in cells grown at 22°C is 10 times higher than that in cells grown at 34°C up