Any feedback?
Literature summary extracted from
- The involvement of beta-amyrin 28-oxidase (Cyp716a52v2) in oleanane-type ginsenoside biosynthesis in Panax ginseng (2013), Plant Cell Physiol., 54, 2034-2046.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.126 | coexpression of PNY1 and CYP716A52v2 in yeast. Transgenic Panax ginseng plants constitutively overexpressing CYP716A52v2 and expressing a CYP716A52v2-RNAi are generated using Agrobacterium tumefaciens mediated genetic transformation | Panax ginseng |
| 1.14.14.126 | expression in Saccharomyces cerevisiae | Panax ginseng |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.126 | 54000 | - |
x * 54000, calculated | Panax ginseng |
| 1.14.14.126 | 54000 | - |
x * 54000, calculated from sequence | Panax ginseng |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.126 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | Panax ginseng | the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro | oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.126 | Panax ginseng | I7C6E8 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.126 | additional information | very low levels of mRNA in other organs than rhizome | Panax ginseng | - |
| 1.14.14.126 | rhizome | - |
Panax ginseng | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.126 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Panax ginseng | oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.126 | beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro | Panax ginseng | oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.126 | ? | x * 54000, calculated | Panax ginseng |
| 1.14.14.126 | ? | x * 54000, calculated from sequence | Panax ginseng |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.126 | beta-amyrin 28-oxidase | - |
Panax ginseng |
| 1.14.14.126 | CYP716A52v2 | - |
Panax ginseng |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.126 | 30 | - |
assay at | Panax ginseng |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.126 | 7.4 | - |
assay at | Panax ginseng |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.14.14.126 | Panax ginseng | CYP716A52v2 mRNA does not respond to methyl jasmonate treatment during the entire culture period | additional information |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.126 | metabolism | the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro | Panax ginseng |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
