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Literature summary extracted from

  • Zhou, X.R.; Robert, S.S.; Petrie, J.R.; Frampton, D.M., Mansour, M.P.; Blackburn, S.I.; Nichols, P.D.; Green, A.G.; Singh, S.P.
    Isolation and characterization of genes from the marine microalga Pavlova salina encoding three front-end desaturases involved in docosahexaenoic acid biosynthesis (2007), Phytochemistry, 68, 785-796.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.30 expression in yeast and in Arabidopsis thaliana Rebecca salina
1.14.19.31 expression in yeast and in Arabidopsis Rebecca salina

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.30 Rebecca salina A4KDP0
-
-
1.14.19.31 Rebecca salina A0PJ29
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.30 an (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Rebecca salina a (5Z,8Z,11Z,14Z)-icosatetra-5,8,11,14-tetraenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.30 an (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Rebecca salina a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.31 a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Rebecca salina a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.31 a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Rebecca salina a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.30 PsD5Des
-
 Rebecca salina
1.14.19.31 DELTA4 desaturase
-
 Rebecca salina
1.14.19.31 PsD4Des
-
 Rebecca salina

Expression

EC Number Organism Comment Expression
1.14.19.30 Rebecca salina the expression is reduced when Pavlova salina is grown at 15°C. The ratio of expression of desaturases in samples grown at 25°C and 20°C, is 5times greater for the DELTA4-desaturase than for the DELTA5-desaturase gene down
1.14.19.30 Rebecca salina higher growth temperature increases the expression of front-end desaturase genes up
1.14.19.31 Rebecca salina the expression is reduced when Pavlova salina is grown at 15°C. The ratio of expression of desaturases in samples grown at 25°C and 20°C, is 5times greater for the DELTA4-desaturase than for the DELTA5-desaturase gene down
1.14.19.31 Rebecca salina higher growth temperature increases the expression of front-end desaturase genes up