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Literature summary extracted from

  • Brinkmann-Chen, S.; Cahn, J.K.; Arnold, F.H.
    Uncovering rare NADH-preferring ketol-acid reductoisomerases (2014), Metab. Eng., 26C, 17-22.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.382 expression in Escherichia coli Thermacetogenium phaeum
1.1.1.382 expression in Escherichia coli uncultured archaeon
1.1.1.382 expression in Escherichia coli Desulfosudis oleivorans
1.1.1.382 expression in Escherichia coli Archaeoglobus fulgidus
1.1.1.383 expression in Escherichia coli Metallosphaera sedula
1.1.1.383 expression in Escherichia coli Hydrogenobaculum sp. Y04AAS1
1.1.1.383 expression in Escherichia coli Ignisphaera aggregans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.382 additional information
-
 NADH Km-value for NADH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Thermacetogenium phaeum
1.1.1.382 0.005
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Archaeoglobus fulgidus
1.1.1.382 0.032
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Desulfosudis oleivorans
1.1.1.382 1.1
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate uncultured archaeon
1.1.1.383 additional information
-
 NADH Km-value for NADH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
1.1.1.383 additional information
-
 NADPH Km-value for NADPH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
1.1.1.383 0.024
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
1.1.1.383 0.031
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
1.1.1.383 0.039
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1
1.1.1.383 0.046
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.382 Archaeoglobus fulgidus O28294
-
-
1.1.1.382 Desulfosudis oleivorans
-
-
-
1.1.1.382 Thermacetogenium phaeum
-
-
-
1.1.1.382 uncultured archaeon
-
-
-
1.1.1.383 Hydrogenobaculum sp. Y04AAS1
-
-
-
1.1.1.383 Ignisphaera aggregans
-
-
-
1.1.1.383 Metallosphaera sedula
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.382
-
 Thermacetogenium phaeum
1.1.1.382
-
 uncultured archaeon
1.1.1.382
-
 Desulfosudis oleivorans
1.1.1.382
-
 Archaeoglobus fulgidus
1.1.1.383
-
 Metallosphaera sedula
1.1.1.383
-
 Hydrogenobaculum sp. Y04AAS1
1.1.1.383
-
 Ignisphaera aggregans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.382 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Thermacetogenium phaeum (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
 ?
1.1.1.382 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate uncultured archaeon (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
 ?
1.1.1.382 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Desulfosudis oleivorans (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
 ?
1.1.1.382 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Archaeoglobus fulgidus (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
 ?
1.1.1.383 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Metallosphaera sedula (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
 ?
1.1.1.383 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Hydrogenobaculum sp. Y04AAS1 (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
 ?
1.1.1.383 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Ignisphaera aggregans (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
 ?
1.1.1.383 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Metallosphaera sedula (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
-
 ?
1.1.1.383 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Hydrogenobaculum sp. Y04AAS1 (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
-
 ?
1.1.1.383 (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Ignisphaera aggregans (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.382 0.1
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Archaeoglobus fulgidus
1.1.1.382 0.22
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate uncultured archaeon
1.1.1.382 0.25
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Desulfosudis oleivorans
1.1.1.382 0.46
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Thermacetogenium phaeum
1.1.1.383 0.02
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
1.1.1.383 0.03
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
1.1.1.383 0.06
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
1.1.1.383 0.07
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
1.1.1.383 0.12
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1
1.1.1.383 0.12
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.382 7
-
 assay at Thermacetogenium phaeum
1.1.1.382 7
-
 assay at uncultured archaeon
1.1.1.382 7
-
 assay at Desulfosudis oleivorans
1.1.1.382 7
-
 assay at Archaeoglobus fulgidus
1.1.1.383 7
-
 assay at Metallosphaera sedula
1.1.1.383 7
-
 assay at Hydrogenobaculum sp. Y04AAS1
1.1.1.383 7
-
 assay at Ignisphaera aggregans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.382 NADH specific for NADH. NADH/NADPH ratio of kcat/Km is 13 Archaeoglobus fulgidus
1.1.1.382 NADH specific for NADH. NADH/NADPH ratio of kcat/Km is 152 uncultured archaeon
1.1.1.382 NADH specific for NADH. NADH/NADPH ratio of kcat/Km is 74 Thermacetogenium phaeum
1.1.1.382 NADH specific for NADH. No activity with NADPH Desulfosudis oleivorans
1.1.1.383 NADH the enzyme can use both NADH and NADPH with similar efficiency Metallosphaera sedula
1.1.1.383 NADH the enzyme can use both NADH and NADPH with similar efficiency Hydrogenobaculum sp. Y04AAS1
1.1.1.383 NADH the enzyme can use both NADH and NADPH with similar efficiency Ignisphaera aggregans
1.1.1.383 NADPH the enzyme can use both NADH and NADPH with similar efficiency Metallosphaera sedula
1.1.1.383 NADPH the enzyme can use both NADH and NADPH with similar efficiency Hydrogenobaculum sp. Y04AAS1
1.1.1.383 NADPH the enzyme can use both NADH and NADPH with similar efficiency Ignisphaera aggregans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.382 additional information
-
 NADH kcat/Km for NADH is at least 460 /mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Thermacetogenium phaeum
1.1.1.382 8
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Desulfosudis oleivorans
1.1.1.382 20
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Archaeoglobus fulgidus
1.1.1.382 200
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate uncultured archaeon
1.1.1.383 additional information
-
 NADH kcat/Km-value for NADH is above 20/mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
1.1.1.383 additional information
-
 NADPH kcat/Km-value for NADPH is above 25/mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
1.1.1.383 2.1
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
1.1.1.383 2.5
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
1.1.1.383 2.7
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1
1.1.1.383 3.2
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1