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Literature summary extracted from

  • Goldberg, R.; Meirovitz, A.; Hirshoren, N.; Bulvik, R.; Binder, A.; Rubinstein, A.M.; Elkin, M.
    Versatile role of heparanase in inflammation (2013), Matrix Biol., 32, 234-240.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.166 heparin
-
 Mammalia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.166 extracellular heparanase is the only known mammalian endoglycosidase capable of degrading heparan sulfate glycosaminoglycan, both in extracellular space and within the cells Mammalia
-
-
3.2.1.166 intracellular heparanase is the only known mammalian endoglycosidase capable of degrading heparan sulfate glycosaminoglycan, both in extracellular space and within the cells Mammalia 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.166 8000
-
 1 * 50000 + 1 * 8000, SDS-PAGE Mammalia
3.2.1.166 50000
-
 1 * 50000 + 1 * 8000, SDS-PAGE Mammalia
3.2.1.166 65000
-
 1 * 65000, precursor polypeptide, SDS-PAGE Mammalia

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.166 Mammalia
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.166 proteolytic modification the 65 kDa precursor polypeptide that is enzymatically inactive. Proteolytic activation by cleavage into two fragments Mammalia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.166 carcinoma cell enhanced growth/aggressiveness of numerous cancer cell types following overexpression of heparanase and inhibition of the tumorigenic/metastatic abilities of cancer cells following heparanase gene silencing Mammalia
-
3.2.1.166 colon
-
 Mammalia
-
3.2.1.166 colonic mucosa
-
 Mammalia
-
3.2.1.166 lung
-
 Mammalia
-
3.2.1.166 lung epithelial cell
-
 Mammalia
-
3.2.1.166 neutrophil
-
 Mammalia
-
3.2.1.166 T-lymphocyte activated Mammalia
-

Subunits

EC Number Subunits Comment Organism
3.2.1.166 heterodimer 1 * 50000 + 1 * 8000, SDS-PAGE Mammalia
3.2.1.166 monomer 1 * 65000, precursor polypeptide, SDS-PAGE Mammalia

Synonyms

EC Number Synonyms Comment Organism
3.2.1.166 heparanase-1
-
 Mammalia

Expression

EC Number Organism Comment Expression
3.2.1.166 Mammalia sepsis-associated inflammatory lung disease causes rapid induction of heparanase activity through a TNFlpha-dependent mechanism in pulmonary microvascular endothelial cells. Heparanase induction is also found in biopsies of human inflammatory lung disease up

General Information

EC Number General Information Comment Organism
3.2.1.166 malfunction enhanced growth/aggressiveness of numerous cancer cell types following overexpression of heparanase and inhibition of the tumorigenic/metastatic abilities of cancer cells following heparanase gene silencing Mammalia
3.2.1.166 physiological function the enzyme degrades side chains of heparan sulfate. Versatile role of heparanase in inflammation, detailed overview. In light of the potential tissue damage as a consequence of inappropriate cleavage of heparan sulfate, under physiological conditions heparanase is tightly regulated. Along with posttranslational proteolytic processing regulation of heparanase gene transcription represents an important control mechanism. Role for heparanase located within the cell nuclei in regulating expression of genes involved in shaping of inflammatory phenotype in endothelial and T cells. Key role of heparan sulfate in glycocalyx structure during acute inflammatory lung injury heparanase-mediated degradation and loss of pulmonary endothelial glycocalyx facilitating neutrophil recruitment. The enzyme is involved in colon inflammatory bowel disease through exposure of the endothelial surface and increased availability of adhesion molecules Mammalia