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Literature summary extracted from
- Engineered bifunctional enzymes of endo-1,4-beta-xylanase/endo-1,4-beta-mannanase were constructed for synergistically hydrolyzing hemicellulose (2013), J. Mol. Catal. B, 97, 311-318.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.78 | gene An15g07760, cloning in Escherichia coli, and expression of recombinant chimeric xylanase/mannanase fusion enzymes in Pichia pastoris strain X33 using GAP promoter, secretion to the medium | Aspergillus niger |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.78 | additional information | construction of a chimeric enzyme of xylanase and mannanase with higher catalytic efficiency and improved properties, the two enzymes are fused together with twelve fusion types | Aspergillus niger |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.78 | additional information | - |
additional information | kinetics of recombinant chimeric enzymes, overview | Aspergillus niger |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.78 | Aspergillus niger | A2R6F5 | gene An15g07760 | - |
| 3.2.1.78 | Aspergillus niger CBS 513.88 | A2R6F5 | gene An15g07760 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.78 | glycoprotein | deglycosylation by endo-beta-N-acetylglucosaminidase H | Aspergillus niger |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.78 | recombinant chimeric enzymes from Pichia pastoris strain X33 culture supernatant by ammonium sulfate fractionation and gel filtration | Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 768.5 | 1249 | purified recombinant chimeric enzymes, pH 5.0, 50°C | Aspergillus niger |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.78 | Beta-mannanase | - |
Aspergillus niger |
| 3.2.1.78 | endo-1,4-beta-mannanase | - |
Aspergillus niger |
| 3.2.1.78 | Man | - |
Aspergillus niger |
| 3.2.1.78 | mannan endo-1,4-beta-mannanase | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 45 | 55 | recombinant chimeric enzymes | Aspergillus niger |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 10 | 80 | activity range, recombinant chimeric enzymes, profiles overview | Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 10 | 80 | varying residual activities of recombinant chimeric enzymes, overview | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 5 | - |
recombinant chimeric enzymes | Aspergillus niger |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 2 | 7 | activity range, recombinant chimeric enzymes, profiles overview | Aspergillus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 2.2 | 11 | purified recombinant chimeric enzymes display similar high pH stability as the wild-type mannanase with over 80% activity in this range, profiles overview | Aspergillus niger |
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Release 2023.1 (January 2023)
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