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Literature summary extracted from
- Functional analysis of a subtilisin-like serine protease gene from biocontrol fungus Trichoderma harzianum (2014), J. Microbiol., 52, 129-138.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.62 | gene ThSS45, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative expression analysis, recombinant expression in in Escherichia coli strain BL21 | Trichoderma harzianum |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.62 | extracellular | - |
Trichoderma harzianum | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 44900 | - |
x * 44900, about, sequence calculation, x * 69000, recombinant enzyme, SDS-PAGE | Trichoderma harzianum |
| 3.4.21.62 | 69000 | - |
x * 44900, about, sequence calculation, x * 69000, recombinant enzyme, SDS-PAGE | Trichoderma harzianum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.62 | Trichoderma harzianum | S5S2B8 | gene ThSS45 | - |
| 3.4.21.62 | Trichoderma harzianum ACCC30371 | S5S2B8 | gene ThSS45 | - |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | ? | x * 44900, about, sequence calculation, x * 69000, recombinant enzyme, SDS-PAGE | Trichoderma harzianum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | subtilisin-like serine protease | - |
Trichoderma harzianum |
| 3.4.21.62 | ThSS45 | - |
Trichoderma harzianum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 40 | - |
recombinant enzyme | Trichoderma harzianum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.62 | 7 | - |
recombinant enzyme | Trichoderma harzianum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.21.62 | Trichoderma harzianum | sequence calculation | - |
5.91 |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.4.21.62 | Trichoderma harzianum | transcription of gene ThSS45 is up-regulated when grown in mineral medium, under carbon starvation, and nitrogen starvation, and in the presence of 1% root powder (3.5fold), 1% stem powder, and 1% leaf powder derived from Populus davidiana × P. bolleana (Shanxin poplar) aseptic seedlings. Gene transcription of ThSS45 is also slightly up-regulated by 1% Alternaria alternata cell wall and 5% Alternaria alternata fermentation liquid | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.4.21.62 | evolution | the enzyme is a member of the peptidases_S8/PCSK9/proteinase K-like family (Pf00082) | Trichoderma harzianum |
| 3.4.21.62 | physiological function | the serine protease may be involved in both mycoparasitism and antibiotic secretion | Trichoderma harzianum |
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Release 2023.1 (January 2023)
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