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Literature summary extracted from

  • Ishibashi, Y.; Kobayashi, U.; Hijikata, A.; Sakaguchi, K.; Goda, H.M.; Tamura, T.; Okino, N.; Ito, M.
    Preparation and characterization of EGCase I, applicable to the comprehensive analysis of GSLs, using a rhodococcal expression system (2012), J. Lipid Res., 53, 2242-2251.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.123 dimethylformamide the activity is enhanced by addition of 5% (v/v) dimethylformamide Rhodococcus equi
3.2.1.123 Dimethylsulfoxide the activity is enhanced by addition of 10% (v/v) dimethylsulfoxide Rhodococcus equi
3.2.1.123 tetrahydrofuran the activity is enhanced by addition of 5% (v/v) tetrahydrofuran Rhodococcus equi
3.2.1.123 Triton X-100 the activity is enhanced by addition of 0.1% (v/v) Triton X-100 Rhodococcus equi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.123 expressed in Rhodococcus erythropolis JCM3201 cells Rhodococcus equi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.123 0.23
-
 ganglioside Gb3Cer isoform EHCase II, pH 5.5 and 37°C Rhodococcus equi
3.2.1.123 0.4
-
 ganglioside GM1a isoform EHCase I, pH 5.5 and 37°C Rhodococcus equi
3.2.1.123 0.43
-
 ganglioside GM1a isoform EHCase II, pH 5.5 and 37°C Rhodococcus equi
3.2.1.123 0.46
-
 ganglioside Gb3Cer isoform EHCase I, pH 5.5 and 37°C Rhodococcus equi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.123 55000
-
 x * 55000, isoform EGCase I, SDS-PAGE Rhodococcus equi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.123 oligoglycosylglucosyl-(1<->1)-ceramide + H2O Rhodococcus equi
-
 ceramide + oligoglycosylglucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.123 Rhodococcus equi
-
 formerly Rhodococcus sp. strain M-750
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.123 Ni Sepharose 6 column chromatography Rhodococcus equi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.123 alpha-L-fucosyl-(1->2)-ganglioside GM1a + H2O isoform EGCase I exhibits 100% activity with this substrate Rhodococcus equi ?
-
 ?
3.2.1.123 Forssman glycolipid + H2O isoform EGCase I exhibits 25.2% activity with this substrate Rhodococcus equi ?
-
 ?
3.2.1.123 ganglioside Gb3Cer + H2O isoform EGCase I exhibits 100% activity with this substrate Rhodococcus equi ?
-
 ?
3.2.1.123 ganglioside Gb4Cer + H2O isoform EGCase I exhibits 33.9% activity with this substrate Rhodococcus equi ?
-
 ?
3.2.1.123 ganglioside GD1a + H2O isoform EGCase I exhibits 100% activity with this substrate Rhodococcus equi ?
-
 ?
3.2.1.123 ganglioside GD1b + H2O isoform EGCase I exhibits 100% activity with this substrate Rhodococcus equi ?
-
 ?
3.2.1.123 ganglioside GD3 + H2O isoform EGCase I exhibits 100% activity with this substrate Rhodococcus equi ?
-
 ?
3.2.1.123 ganglioside GM1a + H2O isoform EGCase I exhibits 100% activity with this substrate Rhodococcus equi ?
-
 ?
3.2.1.123 ganglioside GM3 + H2O isoform EGCase I exhibits 100% activity with this substrate Rhodococcus equi ?
-
 ?
3.2.1.123 glucosylceramide + H2O isoform EGCase I exhibits 6.7% activity with this substrate Rhodococcus equi ?
-
 ?
3.2.1.123 lactosylceramide + H2O isoform EGCase I exhibits 29.9% activity with this substrate Rhodococcus equi ?
-
 ?
3.2.1.123 additional information almost no hydrolysis is observed for isoform EGCase II with alpha-L-fucosyl-(1->2)-ganglioside GM1a, ganglioside Gb3Cer, and ganglioside Gb4Cer. Isoform EGCase I shows no activity with ganglioside GM4, trigalactosylceramide, sulfatide, and sphingomyelin Rhodococcus equi ?
-
 ?
3.2.1.123 additional information for transglycosylation activity, isoform EGCase I prefers alkanols with a relatively long carbon chain such as pentanol and hexanol as an acceptor substrate Rhodococcus equi ?
-
 ?
3.2.1.123 oligoglycosylglucosyl-(1<->1)-ceramide + H2O
-
 Rhodococcus equi ceramide + oligoglycosylglucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.123 ? x * 55000, isoform EGCase I, SDS-PAGE Rhodococcus equi

Synonyms

EC Number Synonyms Comment Organism
3.2.1.123 EGCase I isoform Rhodococcus equi
3.2.1.123 EGCase II isoform Rhodococcus equi
3.2.1.123 endoglycoceramidase
-
 Rhodococcus equi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.123 0.069
-
 ganglioside Gb3Cer isoform EHCase II, pH 5.5 and 37°C Rhodococcus equi
3.2.1.123 5.7
-
 ganglioside GM1a isoform EHCase II, pH 5.5 and 37°C Rhodococcus equi
3.2.1.123 85
-
 ganglioside Gb3Cer isoform EHCase I, pH 5.5 and 37°C Rhodococcus equi
3.2.1.123 120
-
 ganglioside GM1a isoform EHCase I, pH 5.5 and 37°C Rhodococcus equi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.123 5.5 6 isoform EGCase I Rhodococcus equi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.123 0.3
-
 ganglioside Gb3Cer isoform EHCase II, pH 5.5 and 37°C Rhodococcus equi
3.2.1.123 13
-
 ganglioside GM1a isoform EHCase II, pH 5.5 and 37°C Rhodococcus equi
3.2.1.123 190
-
 ganglioside Gb3Cer isoform EHCase I, pH 5.5 and 37°C Rhodococcus equi
3.2.1.123 320
-
 ganglioside GM1a isoform EHCase I, pH 5.5 and 37°C Rhodococcus equi