Literature summary extracted from
Jia, X.; Kwon, S.; Wang, C.I.; Huang, Y.H.; Chan, L.Y.; Tan, C.C.; Rosengren, K.J.; Mulvenna, J.P.; Schroeder, C.I.; Craik, D.J.
Semienzymatic cyclization of disulfide-rich peptides using sortase A (2014), J. Biol. Chem., 289, 6627-6638.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.4.22.70 |
synthesis |
semienzymatic cyclization of disulfide-rich peptides using sortase A, overview |
Staphylococcus aureus |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.22.70 |
gene srtA, recombinant expression of His6-tagged enzyme catalytic domain, comprising residues 60-206, in Escherichia coli strain BL21(DE3) |
Staphylococcus aureus |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.22.70 |
Ca2+ |
required |
Staphylococcus aureus |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.4.22.70 |
additional information |
Staphylococcus aureus |
the enzyme recognizes a 5-residue sequence motif, LPXTG, and cleaves the peptide bond between the Thr and Gly residues, forming an acyl enzyme intermediate between a cysteine at the active site of SrtA and the carboxylate at the truncated C-terminus of the substrate |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.22.70 |
Staphylococcus aureus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.22.70 |
recombinant His6-tagged enzyme catalytic domain from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatograph |
Staphylococcus aureus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.22.70 |
additional information |
the enzyme recognizes a 5-residue sequence motif, LPXTG, and cleaves the peptide bond between the Thr and Gly residues, forming an acyl enzyme intermediate between a cysteine at the active site of SrtA and the carboxylate at the truncated C-terminus of the substrate |
Staphylococcus aureus |
? |
- |
? |
|
3.4.22.70 |
additional information |
semienzymatic cyclization of disulfide-rich peptides using sortase A, including the cyclotide kalata B1, alpha-conotoxin Vc1.1, and sunflower trypsin inhibitor 1, without the need for a thioester linker and using Fmoc chemistry, NMR spectral structure analysis, overview |
Staphylococcus aureus |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.22.70 |
SrtA |
- |
Staphylococcus aureus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.4.22.70 |
37 |
- |
assay at |
Staphylococcus aureus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.22.70 |
7.5 |
- |
assay at |
Staphylococcus aureus |