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Literature summary extracted from

  • Jia, X.; Kwon, S.; Wang, C.I.; Huang, Y.H.; Chan, L.Y.; Tan, C.C.; Rosengren, K.J.; Mulvenna, J.P.; Schroeder, C.I.; Craik, D.J.
    Semienzymatic cyclization of disulfide-rich peptides using sortase A (2014), J. Biol. Chem., 289, 6627-6638.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.22.70 synthesis semienzymatic cyclization of disulfide-rich peptides using sortase A, overview Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.70 gene srtA, recombinant expression of His6-tagged enzyme catalytic domain, comprising residues 60-206, in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.70 Ca2+ required Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.70 additional information Staphylococcus aureus the enzyme recognizes a 5-residue sequence motif, LPXTG, and cleaves the peptide bond between the Thr and Gly residues, forming an acyl enzyme intermediate between a cysteine at the active site of SrtA and the carboxylate at the truncated C-terminus of the substrate ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.70 recombinant His6-tagged enzyme catalytic domain from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatograph Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.70 additional information the enzyme recognizes a 5-residue sequence motif, LPXTG, and cleaves the peptide bond between the Thr and Gly residues, forming an acyl enzyme intermediate between a cysteine at the active site of SrtA and the carboxylate at the truncated C-terminus of the substrate Staphylococcus aureus ?
-
?
3.4.22.70 additional information semienzymatic cyclization of disulfide-rich peptides using sortase A, including the cyclotide kalata B1, alpha-conotoxin Vc1.1, and sunflower trypsin inhibitor 1, without the need for a thioester linker and using Fmoc chemistry, NMR spectral structure analysis, overview Staphylococcus aureus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.70 SrtA
-
Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.70 37
-
assay at Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.70 7.5
-
assay at Staphylococcus aureus