Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Scranton, M.A.; Yee, A.; Park, S.Y.; Walling, L.L.
    Plant leucine aminopeptidases moonlight as molecular chaperones to alleviate stress-induced damage (2012), J. Biol. Chem., 287, 18408-18417.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 expressed in Escherichia coli as a His-tagged fusion protein Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.1 D347N mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity Solanum lycopersicum
3.4.11.1 D347R inactive mutant with disrupted hexameric structure has increased chaperone activity Solanum lycopersicum
3.4.11.1 E429R inactive mutant with disrupted hexameric structure has increased chaperone activity Solanum lycopersicum
3.4.11.1 E429V mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity Solanum lycopersicum
3.4.11.1 K354E inactive mutant with disrupted hexameric structure has increased chaperone activity Solanum lycopersicum
3.4.11.1 K354R mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity Solanum lycopersicum
3.4.11.1 additional information LAP-A exhibits chaperone activity which is independant of its peptidase activity Solanum lycopersicum
3.4.11.1 R431A mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 chloroplast
-
 Solanum lycopersicum 9507
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Solanum lycopersicum Q10712
-
-
3.4.11.1 Solanum lycopersicum Q8GZD8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 using Ni-NTA chromatography Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Solanum lycopersicum L-Leu + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 hexamer disruption of hexameric structure increases in vitro chaperone activity Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP-A stress-induced acidic LAPs, LAP-A exhibits chaperone activity Solanum lycopersicum
3.4.11.1 LAP-N neutral LAP, LAP-N exhibtis chaperone activity Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
 assay at Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8
-
 assay at Solanum lycopersicum

General Information

EC Number General Information Comment Organism
3.4.11.1 malfunction disruption of the LAP-A hexameric structure increases chaperone activity Solanum lycopersicum
3.4.11.1 physiological function LAP-A exhibits chaperone activity which is independant of its peptidase activity Solanum lycopersicum