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Literature summary extracted from

  • Wiethaus, J.; Busch, A.; Kock, K.; Leichert, L.; Herrmann, C.; Frankenberg-Dinkel, N.
    CpeS is a lyase specific for attachment of 3Z-PEB to Cys82 of beta-phycoerythrin from Prochlorococcus marinus MED4 (2010), J. Biol. Chem., 285, 37561-37569.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.29
-
 Prochlorococcus marinus subsp. pastoris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.29 23300
-
 2 * 23300, calculated Prochlorococcus marinus subsp. pastoris
4.4.1.29 47100
-
 gel filtration Prochlorococcus marinus subsp. pastoris

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.29 Prochlorococcus marinus subsp. pastoris Q7V2Z2
-
-
4.4.1.29 Prochlorococcus marinus subsp. pastoris MED4 Q7V2Z2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.29 apo-[phycoerythrin beta-subunit] + 3Z-phycoerythrobilin
-
 Prochlorococcus marinus subsp. pastoris [phycoerythrin beta-subunit]-Cys82-phycoerythrobilin isoform CpeS is specific for 3Z-phycoerythrobilin transfer to Cys82 of phycoerythrin and essential for the correct configuration of the attachment product ?
4.4.1.29 apo-[phycoerythrin beta-subunit] + 3Z-phycoerythrobilin
-
 Prochlorococcus marinus subsp. pastoris MED4 [phycoerythrin beta-subunit]-Cys82-phycoerythrobilin isoform CpeS is specific for 3Z-phycoerythrobilin transfer to Cys82 of phycoerythrin and essential for the correct configuration of the attachment product ?
4.4.1.29 additional information dimeric isoform CpeS is able to bind two molecules of precursor 15,16-dihydrobiliverdin, 3E-phycoerythrobilin, or 3Z-phycoerythrobilin with similar binding kinetics and high affinity. CpeS dimer formation occurs prior to bilin binding. CpeS transfers phycoerythrobilin but not 15,16-dihydrobiliverdin to dimeric phycoerythrin. Only CpeS 3Z-phycoerythrobilin-mediated reactions result in formation of holo-phycoerythrin with identical spectroscopic properties as native-phycoerythrin Prochlorococcus marinus subsp. pastoris ?
-
 ?
4.4.1.29 additional information dimeric isoform CpeS is able to bind two molecules of precursor 15,16-dihydrobiliverdin, 3E-phycoerythrobilin, or 3Z-phycoerythrobilin with similar binding kinetics and high affinity. CpeS dimer formation occurs prior to bilin binding. CpeS transfers phycoerythrobilin but not 15,16-dihydrobiliverdin to dimeric phycoerythrin. Only CpeS 3Z-phycoerythrobilin-mediated reactions result in formation of holo-phycoerythrin with identical spectroscopic properties as native-phycoerythrin Prochlorococcus marinus subsp. pastoris MED4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.29 dimer 2 * 23300, calculated Prochlorococcus marinus subsp. pastoris