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Literature summary extracted from

  • Qiu, X.; Hong, H.; MacKenzie, S.L.
    Identification of a DELTA4 fatty acid desaturase from Thraustochytrium sp. involved in the biosynthesis of docosahexanoic acid by heterologous expression in Saccharomyces cerevisiae and Brassica juncea (2001), J. Biol. Chem., 276, 31561-31566.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.31 heterologous expression in Saccharomyces cerevisiae and Brassica juncea respectively. Introduction of the full-length enzyme into Brassica juncea under the control of the 35S promoter results in the production of (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaeno in leaves when exogenously supplied with (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoic acid Thraustochytrium sp.
1.14.19.43 expressed in Saccharomyces cerevisiae and Brassica juncea Thraustochytrium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.31 49800
-
 x * 49800, calculated fropm sequence Thraustochytrium sp.
1.14.19.43 59100
-
 x * 59100, calculated from amino acid sequence Thraustochytrium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.43 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Thraustochytrium sp.
-
 1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.43 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Thraustochytrium sp. ATCC 21685
-
 1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.31 Thraustochytrium sp. Q8S3C0
-
-
1.14.19.31 Thraustochytrium sp. ATCC 21685 Q8S3C0
-
-
1.14.19.43 Thraustochytrium sp. Q853C0
-
-
1.14.19.43 Thraustochytrium sp. ATCC 21685 Q853C0
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.19.31 a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O (1) Thraustochytrium sp.
a
1.14.19.31 a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O (1) Thraustochytrium sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.31 a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Thraustochytrium sp. a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.31 a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Thraustochytrium sp. ATCC 21685 a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.31 a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Thraustochytrium sp. a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.31 a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Thraustochytrium sp. ATCC 21685 a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.43 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Thraustochytrium sp. 1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.43 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Thraustochytrium sp. ATCC 21685 1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.43 22:4(n-6) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Thraustochytrium sp. docosa-4,7,10,13,16-pentaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.43 22:4(n-6) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Thraustochytrium sp. ATCC 21685 docosa-4,7,10,13,16-pentaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.43 22:5(n-3) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Thraustochytrium sp. docosa-4,7,10,13,16,19-hexaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.43 22:5(n-3) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Thraustochytrium sp. ATCC 21685 docosa-4,7,10,13,16,19-hexaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.31 ? x * 49800, calculated fropm sequence Thraustochytrium sp.
1.14.19.43 ? x * 59100, calculated from amino acid sequence Thraustochytrium sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.19.31 DELTA4 fatty acid desaturase
-
 Thraustochytrium sp.
1.14.19.43 DELTA4 fatty acid desaturase
-
 Thraustochytrium sp.
1.14.19.43 FAD4
-
 Thraustochytrium sp.

General Information

EC Number General Information Comment Organism
1.14.19.31 metabolism the enzyme is involved in the biosynthesis of docosahexanoic acid Thraustochytrium sp.