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Literature summary extracted from

  • Seebeck, F.P.
    In vitro reconstitution of Mycobacterial ergothioneine biosynthesis (2010), J. Am. Chem. Soc., 132, 6632-6633.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.50
-
Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.50 EDTA
-
Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.50 Fe2+ requires Fe2+ for activity Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.118 24139
-
x * 24139, calculated from sequence Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.50 hercynine + gamma-L-glutamyl-L-cysteine + O2 Mycolicibacterium smegmatis the enzyme is part of the biosynthesis pathway of ergothioneine gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
?
3.5.1.118 S-(hercyn-2-yl)-gamma-L-glutamyl-L-cysteine S-oxide + H2O Mycolicibacterium smegmatis the enzyme is part of the biosynthesis pathway of ergothioneine in mycobacteria S-(hercyn-2-yl)-L-cysteine S-oxide + L-glutamate
-
?
3.5.1.118 S-(hercyn-2-yl)-gamma-L-glutamyl-L-cysteine S-oxide + H2O Mycolicibacterium smegmatis ATCC 700084 the enzyme is part of the biosynthesis pathway of ergothioneine in mycobacteria S-(hercyn-2-yl)-L-cysteine S-oxide + L-glutamate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.50 Mycolicibacterium smegmatis A0R5N0
-
-
3.5.1.118 Mycolicibacterium smegmatis A0R5M9
-
-
3.5.1.118 Mycolicibacterium smegmatis ATCC 700084 A0R5M9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.50 hercynine + gamma-L-glutamyl-L-cysteine + O2 the enzyme is part of the biosynthesis pathway of ergothioneine Mycolicibacterium smegmatis gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
?
1.14.99.50 hercynine + gamma-L-glutamyl-L-cysteine + O2 hercynine i.e. Nalpha,Nalpha,Nalpha-trimethyl-L-histidine. The enzyme is specific for both hercynine and gamma-L-glutamyl-L-cysteine. No activity with cysteine, N-acetylcysteine, or glutathione Mycolicibacterium smegmatis gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
?
3.5.1.118 S-(hercyn-2-yl)-gamma-L-glutamyl-L-cysteine S-oxide + H2O
-
Mycolicibacterium smegmatis S-(hercyn-2-yl)-L-cysteine S-oxide + L-glutamate
-
?
3.5.1.118 S-(hercyn-2-yl)-gamma-L-glutamyl-L-cysteine S-oxide + H2O the enzyme is part of the biosynthesis pathway of ergothioneine in mycobacteria Mycolicibacterium smegmatis S-(hercyn-2-yl)-L-cysteine S-oxide + L-glutamate
-
?
3.5.1.118 S-(hercyn-2-yl)-gamma-L-glutamyl-L-cysteine S-oxide + H2O
-
Mycolicibacterium smegmatis ATCC 700084 S-(hercyn-2-yl)-L-cysteine S-oxide + L-glutamate
-
?
3.5.1.118 S-(hercyn-2-yl)-gamma-L-glutamyl-L-cysteine S-oxide + H2O the enzyme is part of the biosynthesis pathway of ergothioneine in mycobacteria Mycolicibacterium smegmatis ATCC 700084 S-(hercyn-2-yl)-L-cysteine S-oxide + L-glutamate
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.118 ? x * 24139, calculated from sequence Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
1.14.99.50 EgtB
-
Mycolicibacterium smegmatis
3.5.1.118 EgtC
-
Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.50 7.6
-
assay at Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
1.14.99.50 metabolism the enzyme is part of the biosynthesis pathway of ergothioneine Mycolicibacterium smegmatis
3.5.1.118 metabolism the enzyme is part of the biosynthesis pathway of ergothioneine in mycobacteria Mycolicibacterium smegmatis