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Literature summary extracted from

  • Katrolia, P.; Yan, Q.; Zhang, P.; Zhou, P.; Yang, S.; Jiang, Z.
    Gene cloning and enzymatic characterization of an alkali-tolerant endo-1,4-beta-mannanase from Rhizomucor miehei (2013), J. Agric. Food Chem., 61, 394-401.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.78 industry the enzyme is highly useful in the food/feed, paper and pulp, and detergent industries Rhizomucor miehei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 gene RmMan5A, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21 Rhizomucor miehei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
additional information Km values of the recombinant enzyme are 3.78 mg/ml for locust bean gum, and 7.75 mg/ml for guar gum Rhizomucor miehei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 43000
-
x * 43000, recombinant enzyme, SDS-PAGE Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Rhizomucor miehei
-
gene RmMan5A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Rhizomucor miehei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 10760
-
purified recombinant enzyme, pH 7.0, 55°C, substrate locust bean gum Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 guar gum + H2O
-
Rhizomucor miehei mannobiose + mannotriose mannobiose, mannotriose, and a mixture of various mannose-linked oligosaccharides ?
3.2.1.78 locust bean gum + H2O best substrate Rhizomucor miehei mannobiose + mannotriose mannobiose, mannotriose, and a mixture of various mannose-linked oligosaccharides ?
3.2.1.78 additional information the enzyme shows requirement of four mannose residues for hydrolysis, and is also capable of catalyzing transglycosylation reactions Rhizomucor miehei ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 43000, recombinant enzyme, SDS-PAGE Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 endo-1,4-beta-mannanase
-
Rhizomucor miehei
3.2.1.78 endo-acting beta-1,4-mannanase
-
Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 55
-
-
Rhizomucor miehei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 30 80 activity range, recombinant enzyme, profile overview Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 55
-
purified recombinant enzyme, without substrate, stable up to, 90% activity remaning at 55°C after 30 min Rhizomucor miehei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.78 4.77
-
guar gum pH 7.0, 55°C, recombinant enzyme Rhizomucor miehei
3.2.1.78 14.9
-
locust bean gum pH 7.0, 55°C, recombinant enzyme Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 7
-
-
Rhizomucor miehei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 4 10 activity range, recombinant enzyme, profile overview Rhizomucor miehei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 4 10 purified recombinant enzyme, 30 min, without substrate, over 80% activity remaining Rhizomucor miehei