Literature summary extracted from
Katrolia, P.; Yan, Q.; Zhang, P.; Zhou, P.; Yang, S.; Jiang, Z.
Gene cloning and enzymatic characterization of an alkali-tolerant endo-1,4-beta-mannanase from Rhizomucor miehei (2013), J. Agric. Food Chem., 61, 394-401.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.2.1.78 |
industry |
the enzyme is highly useful in the food/feed, paper and pulp, and detergent industries |
Rhizomucor miehei |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.78 |
gene RmMan5A, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21 |
Rhizomucor miehei |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.2.1.78 |
additional information |
- |
additional information |
Km values of the recombinant enzyme are 3.78 mg/ml for locust bean gum, and 7.75 mg/ml for guar gum |
Rhizomucor miehei |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.78 |
43000 |
- |
x * 43000, recombinant enzyme, SDS-PAGE |
Rhizomucor miehei |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.78 |
Rhizomucor miehei |
- |
gene RmMan5A |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.78 |
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography |
Rhizomucor miehei |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.2.1.78 |
10760 |
- |
purified recombinant enzyme, pH 7.0, 55°C, substrate locust bean gum |
Rhizomucor miehei |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.78 |
guar gum + H2O |
- |
Rhizomucor miehei |
mannobiose + mannotriose |
mannobiose, mannotriose, and a mixture of various mannose-linked oligosaccharides |
? |
|
3.2.1.78 |
locust bean gum + H2O |
best substrate |
Rhizomucor miehei |
mannobiose + mannotriose |
mannobiose, mannotriose, and a mixture of various mannose-linked oligosaccharides |
? |
|
3.2.1.78 |
additional information |
the enzyme shows requirement of four mannose residues for hydrolysis, and is also capable of catalyzing transglycosylation reactions |
Rhizomucor miehei |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.78 |
? |
x * 43000, recombinant enzyme, SDS-PAGE |
Rhizomucor miehei |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.78 |
endo-1,4-beta-mannanase |
- |
Rhizomucor miehei |
3.2.1.78 |
endo-acting beta-1,4-mannanase |
- |
Rhizomucor miehei |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.1.78 |
55 |
- |
- |
Rhizomucor miehei |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.2.1.78 |
30 |
80 |
activity range, recombinant enzyme, profile overview |
Rhizomucor miehei |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.2.1.78 |
55 |
- |
purified recombinant enzyme, without substrate, stable up to, 90% activity remaning at 55°C after 30 min |
Rhizomucor miehei |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
3.2.1.78 |
4.77 |
- |
guar gum |
pH 7.0, 55°C, recombinant enzyme |
Rhizomucor miehei |
|
3.2.1.78 |
14.9 |
- |
locust bean gum |
pH 7.0, 55°C, recombinant enzyme |
Rhizomucor miehei |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.78 |
7 |
- |
- |
Rhizomucor miehei |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.2.1.78 |
4 |
10 |
activity range, recombinant enzyme, profile overview |
Rhizomucor miehei |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
3.2.1.78 |
4 |
10 |
purified recombinant enzyme, 30 min, without substrate, over 80% activity remaining |
Rhizomucor miehei |