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Literature summary extracted from

  • Lacombe-Harvey, M.E.; Fortin, M.; Ohnuma, T.; Fukamizo, T.; Letzel, T.; Brzezinski, R.
    A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding (2013), BMC Biochem., 14, 23.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 expressed in Streptomyces lividans strain TK24 and Escherichia coli Gold cells Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.132 D40G the mutant shows drastic loss of activity Streptomyces sp.
3.2.1.132 D40G/R42E the mutant shows drastic loss of activity Streptomyces sp.
3.2.1.132 D40G/R42K the mutant shows drastic loss of activity Streptomyces sp.
3.2.1.132 R42E the mutant shows drastic loss of activity Streptomyces sp.
3.2.1.132 R42K the mutant shows drastic loss of activity Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 chitosan the wild type enzyme shows a Ki value of 1.547 mg/ml Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Streptomyces sp. the wild type chitosanase shows a clear preference for chitosan with high degrees of N-deacetylation (84-97%) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Streptomyces sp. P33665
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 0.0012
-
 mutant enzyme D40G/R42E, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
3.2.1.132 0.018
-
 mutant enzyme D40G/R42K, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
3.2.1.132 0.9
-
 mutant enzyme R42E at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
3.2.1.132 1.4
-
 mutant enzyme R42K, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
3.2.1.132 1.6
-
 mutant enzyme D40G, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
3.2.1.132 52.9
-
 wild type enzyme, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O the wild type chitosanase shows a clear preference for chitosan with high degrees of N-deacetylation (84-97%) Streptomyces sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 Csn
-
 Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.132 0.9
-
 chitosan mutant enzyme R42K, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
3.2.1.132 1.6
-
 chitosan mutant enzyme R42E at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
3.2.1.132 10.7
-
 chitosan wild type enzyme, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5.5
-
-
 Streptomyces sp.