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Literature summary extracted from

  • Hirano, K.; Arayaveerasid, S.; Seki, K.; Adams, D.J.; Mitsutomi, M.
    Characterization of a chitosanase from Aspergillus fumigatus ATCC13073 (2012), Biosci. Biotechnol. Biochem., 76, 1523-1528.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.132 1.2
-
 GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN at pH 5.7 and 37°C Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 23525
-
 x * 23525, MALDI-TOF mass spectrometry Aspergillus fumigatus
3.2.1.132 30000
-
 x * 30000, SDS-PAGE Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Aspergillus fumigatus the enzyme hydrolyzes 70% deacetylated chitosan faster than fully deacetylated chitosan ?
-
 ?
3.2.1.132 chitosan + H2O Aspergillus fumigatus ATCC 13073 the enzyme hydrolyzes 70% deacetylated chitosan faster than fully deacetylated chitosan ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Aspergillus fumigatus
-
-
-
3.2.1.132 Aspergillus fumigatus ATCC 13073
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 CM Sephadex C50 gel filtration and Sephadex G-100 gel filtration Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 7.59
-
 culture filtrate, at pH 5.7 and 37°C Aspergillus fumigatus
3.2.1.132 8.8
-
 after 1.16fold purification, at pH 5.7 and 37°C Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O the enzyme hydrolyzes 70% deacetylated chitosan faster than fully deacetylated chitosan Aspergillus fumigatus ?
-
 ?
3.2.1.132 chitosan + H2O the enzyme hydrolyzes 70% deacetylated chitosan faster than fully deacetylated chitosan Aspergillus fumigatus ATCC 13073 ?
-
 ?
3.2.1.132 Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-GlcN-beta-(1->4) + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.132 Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-GlcN-beta-(1->4) + H2O
-
 Aspergillus fumigatus ATCC 13073 ?
-
 ?
3.2.1.132 GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + H2O best substrate Aspergillus fumigatus GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + GlcN-beta-(1->4)-GlcN + GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4) is the main product ?
3.2.1.132 GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.132 GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.132 GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + H2O
-
 Aspergillus fumigatus ATCC 13073 ?
-
 ?
3.2.1.132 additional information the enzyme does not hydrolyze colloidal chitin or carboxymethylcellulose Aspergillus fumigatus ?
-
 ?
3.2.1.132 additional information the enzyme splits GlcN-GlcN and GlcNAc-GlcN bonds but not GlcNAc-GlcNAc or GlcN-GlcNAc bonds Aspergillus fumigatus ?
-
 ?
3.2.1.132 additional information the enzyme does not hydrolyze colloidal chitin or carboxymethylcellulose Aspergillus fumigatus ATCC 13073 ?
-
 ?
3.2.1.132 additional information the enzyme splits GlcN-GlcN and GlcNAc-GlcN bonds but not GlcNAc-GlcNAc or GlcN-GlcNAc bonds Aspergillus fumigatus ATCC 13073 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 30000, SDS-PAGE Aspergillus fumigatus
3.2.1.132 ? x * 23525, MALDI-TOF mass spectrometry Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 chitosanase II
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 40
-
-
 Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.132 30 50 about 45% activity at 30°C, about 85% activity at 35°C, 100% activity at 40°C, about 30% activity at 50°C Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 30 80 after 15 min incubation the enzyme shows 100% activity at 30°C, about 90% at 40°C, about 85% at 50°C, about 82% at 60°C, about 80% at 70°C, about 60% at 80°C, about 40° at 90°C, and about 20% activity at 100°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 6
-
-
 Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 1 9 the enzyme is stable and retains more than 80% of its original activity from pH 1.0-9.0 after 15 min Aspergillus fumigatus