EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.11.19 | expression in Aspergillus oryzae | Aspergillus oryzae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.19 | AEBSF | 1 mM, 45% residual activity | Aspergillus oryzae | |
3.4.11.19 | Cd2+ | 1 mM, less than 5% residual activity | Aspergillus oryzae | |
3.4.11.19 | Co2+ | 1 mM, 50% residual activity | Aspergillus oryzae | |
3.4.11.19 | Cu2+ | 1 mM, 5% residual activity | Aspergillus oryzae | |
3.4.11.19 | EDTA | 1 mM, 40% residual activity | Aspergillus oryzae | |
3.4.11.19 | Ni2+ | 1 mM, 20% residual activity | Aspergillus oryzae | |
3.4.11.19 | Zn2+ | 1 mM, less than 5% residual activity | Aspergillus oryzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.19 | 0.25 | - |
Gly 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
3.4.11.19 | 0.33 | - |
D-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
3.4.11.19 | 6.7 | - |
L-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.19 | 59300 | - |
2 * 59300, calculated | Aspergillus oryzae |
3.4.11.19 | 126300 | - |
gel filtration | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.19 | Aspergillus oryzae | G7H7Y1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.19 | recombinant protein | Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.19 | D-Ala 4-nitroanilide + H2O | 93% of the activity with Gly 4-nitroanilide | Aspergillus oryzae | D-Ala + 4-nitroaniline | - |
? | |
3.4.11.19 | Gly 4-nitroanilide + H2O | - |
Aspergillus oryzae | Gly + 4-nitroaniline | - |
? | |
3.4.11.19 | Gly-L-Ala + H2O | - |
Aspergillus oryzae | Gly + L-Ala | - |
? | |
3.4.11.19 | Gly-L-Cys + H2O | - |
Aspergillus oryzae | Gly + L-Cys | - |
? | |
3.4.11.19 | L-Ala 4-nitroanilide + H2O | 12% of the activity with Gly 4-nitroanilide | Aspergillus oryzae | L-Ala + 4-nitroaniline | - |
? | |
3.4.11.19 | L-Ala-D-Ala + H2O | - |
Aspergillus oryzae | L-Ala + D-Ala | - |
? | |
3.4.11.19 | additional information | enzyme shows strict D-stereospecific aminopeptidase activity. No or very poor activity with beta-Ala 4-nitroanilide, L-Leu 4-nitroanilide, D-Leu 4-nitroanilide,L-Met 4-nitroanilide, L-Pro 4-nitroanilide, L-Val 4-nitroanilide, L-Ile 4-nitroanilide, L-Phe 4-nitroanilide, D-Phe 4-nitroanilide, L-Lys 4-nitroanilide, L-Arg 4-nitroanilide, L-His 4-nitroanilide, L-Asp 4-nitroanilide, L-Glu 4-nitroanilide. No substrates: L-Ala-Gly and L-Cys-Gly dipeptides and L-Cys-Gly-Gly tripeptide | Aspergillus oryzae | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.11.19 | dimer | 2 * 59300, calculated | Aspergillus oryzae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.11.19 | GdaA | - |
Aspergillus oryzae |
3.4.11.19 | glycine D-alanine aminopeptidase | - |
Aspergillus oryzae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.19 | 40 | - |
- |
Aspergillus oryzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.19 | 2100 | - |
L-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
3.4.11.19 | 2550 | - |
D-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
3.4.11.19 | 2783 | - |
Gly 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.19 | 8 | 10 | - |
Aspergillus oryzae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.19 | 5 | 11 | - |
Aspergillus oryzae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.19 | 8 | 11 | - |
Aspergillus oryzae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.11.19 | physiological function | no significant differences are observed in the vegetative growth and conidiation on minimal and complete media in the enzyme gene disruptant or overexpression strains | Aspergillus oryzae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.19 | 313 | - |
L-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
3.4.11.19 | 7750 | - |
D-Ala 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae | |
3.4.11.19 | 11000 | - |
Gly 4-nitroanilide | pH 9.0, 30°C | Aspergillus oryzae |