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Literature summary extracted from

  • Marui, J.; Matsushita-Morita, M.; Tada, S.; Hattori, R.; Suzuki, S.; Amano, H.; Ishida, H.; Yamagata, Y.; Takeuchi, M.; Kusumoto, K.
    Enzymatic properties of the glycine D-alanine aminopeptidase of Aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji) (2012), Appl. Microbiol. Biotechnol., 93, 655-669.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.19 expression in Aspergillus oryzae Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.19 AEBSF 1 mM, 45% residual activity Aspergillus oryzae
3.4.11.19 Cd2+ 1 mM, less than 5% residual activity Aspergillus oryzae
3.4.11.19 Co2+ 1 mM, 50% residual activity Aspergillus oryzae
3.4.11.19 Cu2+ 1 mM, 5% residual activity Aspergillus oryzae
3.4.11.19 EDTA 1 mM, 40% residual activity Aspergillus oryzae
3.4.11.19 Ni2+ 1 mM, 20% residual activity Aspergillus oryzae
3.4.11.19 Zn2+ 1 mM, less than 5% residual activity Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.19 0.25
-
 Gly 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
3.4.11.19 0.33
-
 D-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
3.4.11.19 6.7
-
 L-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.19 59300
-
 2 * 59300, calculated Aspergillus oryzae
3.4.11.19 126300
-
 gel filtration Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.19 Aspergillus oryzae G7H7Y1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.19 recombinant protein Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.19 D-Ala 4-nitroanilide + H2O 93% of the activity with Gly 4-nitroanilide Aspergillus oryzae D-Ala + 4-nitroaniline
-
 ?
3.4.11.19 Gly 4-nitroanilide + H2O
-
 Aspergillus oryzae Gly + 4-nitroaniline
-
 ?
3.4.11.19 Gly-L-Ala + H2O
-
 Aspergillus oryzae Gly + L-Ala
-
 ?
3.4.11.19 Gly-L-Cys + H2O
-
 Aspergillus oryzae Gly + L-Cys
-
 ?
3.4.11.19 L-Ala 4-nitroanilide + H2O 12% of the activity with Gly 4-nitroanilide Aspergillus oryzae L-Ala + 4-nitroaniline
-
 ?
3.4.11.19 L-Ala-D-Ala + H2O
-
 Aspergillus oryzae L-Ala + D-Ala
-
 ?
3.4.11.19 additional information enzyme shows strict D-stereospecific aminopeptidase activity. No or very poor activity with beta-Ala 4-nitroanilide, L-Leu 4-nitroanilide, D-Leu 4-nitroanilide,L-Met 4-nitroanilide, L-Pro 4-nitroanilide, L-Val 4-nitroanilide, L-Ile 4-nitroanilide, L-Phe 4-nitroanilide, D-Phe 4-nitroanilide, L-Lys 4-nitroanilide, L-Arg 4-nitroanilide, L-His 4-nitroanilide, L-Asp 4-nitroanilide, L-Glu 4-nitroanilide. No substrates: L-Ala-Gly and L-Cys-Gly dipeptides and L-Cys-Gly-Gly tripeptide Aspergillus oryzae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.19 dimer 2 * 59300, calculated Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.4.11.19 GdaA
-
 Aspergillus oryzae
3.4.11.19 glycine D-alanine aminopeptidase
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.19 40
-
-
 Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.19 2100
-
 L-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
3.4.11.19 2550
-
 D-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
3.4.11.19 2783
-
 Gly 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.19 8 10
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.19 5 11
-
 Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.19 8 11
-
 Aspergillus oryzae

General Information

EC Number General Information Comment Organism
3.4.11.19 physiological function no significant differences are observed in the vegetative growth and conidiation on minimal and complete media in the enzyme gene disruptant or overexpression strains Aspergillus oryzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.19 313
-
 L-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
3.4.11.19 7750
-
 D-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
3.4.11.19 11000
-
 Gly 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae