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Literature summary extracted from

  • Sakuradani, E.; Abe, T.; Iguchi, K.; Shimizu, S.
    A novel fungal omega3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S-4 (2005), Appl. Microbiol. Biotechnol., 66, 648-654.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.25 expression in Saccharomyces cerevisiae Mortierella alpina

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.25 Mortierella alpina Q59J82
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-
1.14.19.25 Mortierella alpina 1S-4 Q59J82
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.25 a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases Mortierella alpina a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.25 a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases Mortierella alpina 1S-4 a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.25 a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ (6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases Mortierella alpina a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O (6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid] ?
1.14.19.25 a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ (6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases Mortierella alpina 1S-4 a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O (6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid] ?
1.14.19.25 a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ (9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases Mortierella alpina an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O (9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid] ?
1.14.19.25 a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ (9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases Mortierella alpina 1S-4 an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O (9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid] ?
1.14.19.25 an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases Mortierella alpina a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.25 an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases Mortierella alpina 1S-4 a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.25 additional information the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity Mortierella alpina ?
-
 ?
1.14.19.25 additional information the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity Mortierella alpina 1S-4 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.25 maw3
-
 Mortierella alpina
1.14.19.25 omega3-desaturase ambiguous Mortierella alpina